JCRB0621 NB-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0621 Cell Name   NB-1
Profile   Human neuroblastoma cell line differentiate in vitro by cAMP. Other Name   NB-1
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   2
Identity   available Tissue for Primary Cancer   neural
Case history    Metastasis   Yes
Tissue Metastasized   lymph node of neck Genetics   neuroblastoma (M)
Life Span   infinite Crisis PDL   
Morphology   round/tear-drop Character   Differentiate by (But)2cAMP (1mM).
Classify   tumor Established by   Miyake,S.
Registered by   Sekiguchi,M. Regulation for Distribution   
Comment   Mycoplasma contaminated when submitted to the bank. Year   1985
Medium   RPMI1640 medium ,45%, with Eagle's minimal essential medium ,45% , and 10% fetal calf serum. Methods for Passages   Mechanical dispersion.
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   neuroblastoma
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1440
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 949179
Reference
Pubmed id:201151Differentiation of human neuroblastoma cells in vitro--morphological changes induced by dibutyrl cyclic AMP.
Ishikawa S
Acta Pathol Jpn. 1977 Sep;27(5):697-711
Pubmed id:4144593Catecholamine metabolism in tissue culture cells of a neuroblastoma.
Imashuku S,Inui A,Nakamura T,Tanaka J,Miyake S
J Clin Endocrinol Metab. 1973 May;36(5):931-6

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LOT Information

Cell No.   JCRB0621 Cell Name   NB-1
LOT No.   02262008 Lot Specification   distribution
Medium   1:1 mixture of Eagle's minimum essential medium and RPMI1640 medium with 10% fetal bovine serum (GIBCO Cat.#10099, lot 484375) Temperature   37 C
Cell Density at Seeding   2 - 3 x 10^5 cells/ml Methods for Passages   mechanical dispersion by pipetting
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)   ca. 70% Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - basal medium CO2 Conc.   5%
Viability immediately after thawing (%)   79% Additional information   
Cell No.   JCRB0621 Cell Name   
LOT No.   120898 Lot Specification   distribution
Medium   45% RPMI1640 + 45% Eagle's minimal essential medium + 10% fetal calf serum (Biowhittaker6M0121) Temperature   37C
Cell Density at Seeding   5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   ca 60hrs Cell Number in Vial (cells/1ml)   3.1 x 10^6
Viability at cell freezing (%)   88% Antibiotics Used   free
Passage Number   P9* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD(type B), LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0621 Cell Name   NB-1
LOT No.   071796 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LDH, G6PD, NP
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0621 Cell Name   NB-1
LOT No.   08152016 Lot Specification   distribution
Medium   1:1 mixture of RPMI-1640 medium and Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat.# 172012) Temperature   37 C
Cell Density at Seeding   0.7 - 1.5 x 10^5 cells/mL Methods for Passages   Mechanical dispersion (pipetting).
Doubling Time   approx. 2 days. Cell Number in Vial (cells/1ml)   2.7 x 10^6
Viability at cell freezing (%)   90.9 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640/EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   
Cell No.   JCRB0621 Cell Name   NB-1
LOT No.   05272024 Lot Specification   distribution
Medium   1:1 mixture of RPMI-1640 medium and Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/mL Methods for Passages   Mechanical dispersion by pipetting.
Doubling Time   approx. 42 hrs. Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   86 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:9,11
D13S317:10,13
D7S820:8,12
D16S539:9
VWA:16,19
TH01:7
AM:X
TPOX:11
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - basal culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   80 Additional information   
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