JCRB0622 HSC-2

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0622 Cell Name   HSC-2
Profile   squamous carcinoma, mouth Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   69
Identity   available Tissue for Primary Cancer   oral
Case history   squamous carcinoma. Metastasis   
Tissue Metastasized    Genetics   
Life Span    Crisis PDL   
Morphology   epithelial-like Character   This cell line has neither invasive nor metastatic potential and grew in synthetic medium PF86-1.
Classify   tumor Established by   Momose,F.
Registered by   Momose,F. Regulation for Distribution   
Comment    Year   1985
Medium   Eagle's minimal essential medium with 10% calf serum. Methods for Passages   Cells are treated with trypsin-EDTA, and dilute 1/8 once a week.
Cell Number on Passage   1 x 10^5 cells/10cm dish Race   
CO2 Conc.   5 % Tissue Sampling   mouth
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1287
Reference
Pubmed id:2228902Growth of the malignant and nonmalignant human squamous cells in a protein-free defined medium.
Rikimaru K,Toda H,Tachikawa N,Kamata N,Enomoto S
In Vitro Cell Dev Biol. 1990 Sep;26(9):849-56
Pubmed id:2585303Variant sublines with different metastatic potentials selected in nude mice from human oral squamous cell carcinomas.
Momose F,Araida T,Negishi A,Ichijo H,Shioda S,Sasaki S
J Oral Pathol Med. 1989 Aug;18(7):391-5

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LOT Information

Cell No.   JCRB0622 Cell Name   
LOT No.   08082012 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% newborn calf serum (GIBCO Cat. # 16010) Temperature   37 C
Cell Density at Seeding   5.4 - 7.7 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 32 hrs Cell Number in Vial (cells/1ml)   6.3 x 10^5
Viability at cell freezing (%)   95.3 Antibiotics Used   free
Passage Number   Unknown (19 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0622 Cell Name   
LOT No.   02172009 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% calf serum (newborn calf serum, GIBCO) Temperature   37 C
Cell Density at Seeding   3 - 4 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time   34 hrs Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   97.5 Antibiotics Used   free
Passage Number   Unknown (19 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0622 Cell Name   
LOT No.   07152004 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   3 - 7 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   27 hrs Cell Number in Vial (cells/1ml)   1.0 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   P19* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0622 Cell Name   HSC-2
LOT No.   070296 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0622 Cell Name   HSC-2
LOT No.   091985 Lot Specification   distribution
Medium   MEM + 10%CS Temperature   37
Cell Density at Seeding   1 X 10^5 Methods for Passages   trypsin-EDTA, 1:8 dilution once a week
Doubling Time   NT Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)   NT Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0622 Cell Name   
LOT No.   012999 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% calf serum Temperature   37 C
Cell Density at Seeding   6-7 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T.=ca. 38 hrs Cell Number in Vial (cells/1ml)   7.8 x 10^5
Viability at cell freezing (%)   94.0 Antibiotics Used   free
Passage Number   P18* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, MD, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0622 Cell Name   HSC-2
LOT No.   06192015 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% newborn calf serum (NBS; GIBCO Cat. # 16010). Temperature   37 C
Cell Density at Seeding   2.0 - 3.7 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 30 hrs. Cell Number in Vial (cells/1ml)   9.4 x 10^5
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   Unknown (19 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,12
D13S317:11,12
D7S820:9,12
D16S539:12
VWA:16,18
TH01:6,7
AM:X,Y
TPOX:8
CSF1PO:12,13
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% newborn calf serum - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   96 Additional information   
Cell No.   JCRB0622 Cell Name   HSC-2
LOT No.   06192017 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% newborn calf serum (NCS; GIBCO Cat. # 16010). Temperature   37 C
Cell Density at Seeding   1.3 - 3.4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 18 hrs. Cell Number in Vial (cells/1ml)   5.6 x 10^5
Viability at cell freezing (%)   98.9 Antibiotics Used   free
Passage Number   Unknown (19 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% NCS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   98.9 Additional information   
Cell No.   JCRB0622 Cell Name   HSC-2
LOT No.   02082021 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% newborn calf serum (NCS; Biowest Cat. # S0750) Temperature   37 C
Cell Density at Seeding   6 - 7 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx 26 hrs. Cell Number in Vial (cells/1ml)   9.8 x 10^5
Viability at cell freezing (%)   98.9 Antibiotics Used   free
Passage Number   Unknown (20 at bank) PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% NCS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   97.0 Additional information   
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