JCRB0623 HSC-3

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0623 Cell Name   HSC-3
Profile   Human oral squamous carcinoma cell line with high metastatic potential. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   64
Identity   available Tissue for Primary Cancer   oral
Case history    Metastasis   
Tissue Metastasized    Genetics   squamous carcinoma, tongue, age 64, (M)
Life Span    Crisis PDL   
Morphology    Character   squamous carcinoma, tongue, age 64, (M)
Classify   tumor Established by   Momose,F.
Registered by   Momose,F. Regulation for Distribution   
Comment    Year   
Medium   Eagle's minimal essential medium with 10% calf serum. Methods for Passages   Cells harvested after 0.25%trypsin and 0.02%EDTA treatment.Subculture with 1/8 dilution once a week.
Cell Number on Passage   1 x 10^5 cells/10cm dish Race   
CO2 Conc.   5 % Tissue Sampling   tongue
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1288
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 907061
Reference
Pubmed id:9813718 Isolation of a highly metastatic cell line to lymph node in human oral squamous cell carcinoma by orthotopic implantation in nude mice.
Matsui T,Ota T,Ueda Y,Tanino M,Odashima S
Oral Oncol. 1998 Jul;34(4):253-6
Pubmed id:2228902 Growth of the malignant and nonmalignant human squamous cells in a protein-free defined medium.
Rikimaru K,Toda H,Tachikawa N,Kamata N,Enomoto S
In Vitro Cell Dev Biol. 1990 Sep;26(9):849-56
Pubmed id:2585303 Variant sublines with different metastatic potentials selected in nude mice from human oral squamous cell carcinomas.
Momose F,Araida T,Negishi A,Ichijo H,Shioda S,Sasaki S
J Oral Pathol Med. 1989 Aug;18(7):391-5

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LOT Information

Cell No.   JCRB0623 Cell Name   HSC-3
LOT No.   07242009 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% newborn calf serum (GIBCO Cat. # 16010) // Ampoule mislabeled as "lot# 07242008" Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^5 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 44 hrs Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   96.3 Antibiotics Used   free
Passage Number   Unknown (12 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,13
D13S317:12
D7S820:13
D16S539:9
VWA:14,17
TH01:6,9.3
AM:X,Y
TPOX:8
CSF1PO:11
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0623 Cell Name   
LOT No.   09032003 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   6 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2.9 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   > P11 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0623 Cell Name   HSC-3
LOT No.   091985 Lot Specification   distribution
Medium   MEM + 10%CS Temperature   37
Cell Density at Seeding   1 X 10^5 Methods for Passages   trypsin-EDTA, 1:8 dilution once a week
Doubling Time   NT Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)   NT Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0623 Cell Name   
LOT No.   121498 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% calf serum Temperature   37 C
Cell Density at Seeding    Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T.=ca. 60 hrs Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   93.0 Antibiotics Used   free
Passage Number   P10* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0623 Cell Name   HSC-3
LOT No.   02022015 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% newborn calf serum (NCS; GIBCO Cat. # 16010) Temperature   37 C
Cell Density at Seeding   6.1 - 7.3 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 52 hrs Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% newborn calf serum - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   94
Additional information   
Cell No.   JCRB0623 Cell Name   HSC-3
LOT No.   07112016 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% newborn calf serum (GIBCO Cat. # 16010). Temperature   37 C
Cell Density at Seeding   1.1 - 1.5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   42 - 65 hrs Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% newborn calf serum - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   98
Additional information   
Cell No.   JCRB0623 Cell Name   HSC-3
LOT No.   06112018 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% newborn calf serum (NCS; Biowest Cat. # S0750) Temperature   37 C
Cell Density at Seeding   1.6 - 1.8 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 37 hrs. Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% newborn calf serum - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   94
Additional information   
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