JCRB0625 Ca9-22

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0625 Cell Name   Ca9-22
Profile   squamous carcinoma, gingiva Other Name   Ca
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   
Identity   available Tissue for Primary Cancer   oral
Case history    Metastasis   
Tissue Metastasized    Genetics   gingival carcinoma
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   gingival carcinoma
Classify    Established by   Horikoshi,M.
Registered by   Rikimaru,K. Regulation for Distribution   Depends on the research.
Comment   EGF receptor is produced extensively. Year   1985
Medium   Eagle's minimal essential medium with 0.6g/l glutamine and 10% fetal calf serum. Methods for Passages   Cells are treated with 0.04% trypsin. 1/20 - 1/30 dilution.
Cell Number on Passage   5x10^4 cells/5ml Race   Japanese
CO2 Conc.   5 % Tissue Sampling   gingival
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1102
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 753538
Reference
Pubmed id:275414[Studies on lactate dehydrogenase isoenzymes in a cell line (Ca 9-22) derived from carcinoma of the gingiva (author's transl)].
Kimura Y
Kokubyo Gakkai Zasshi. 1978 Mar;45(1):20-35
Pubmed id:4549822[A new human cell line derived from human carcinoma of the gingiva. I. Its establishment and morphological studies].
Horikoshi M,Kimura Y,Nagura H,Ono T,Ito H
Nihon Koku Geka Gakkai Zasshi. 1974;20(2):100-6

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LOT Information

Cell No.   JCRB0625 Cell Name   
LOT No.   05182010 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 1.5 x 10^5 cells/ml Methods for Passages   Cells were harvested after treatment with 0.04% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time   approx. 32 hrs Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   95.9 Antibiotics Used   free
Passage Number   Unknown (16 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0625 Cell Name   
LOT No.   04252008 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 2 x L-glutamine (final conc. 0.6 g/L) and 10% fetal bovine serum (FBS Lot; GIBCO Cat. No. 10099, Lot 484375) Temperature   37 C
Cell Density at Seeding   3 - 5 x 10^4 cells/ml Methods for Passages   0.04% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time   19 hrs Cell Number in Vial (cells/1ml)   3.1 x 10^6
Viability at cell freezing (%)   94.0 Antibiotics Used   free
Passage Number   Unknown (16 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0625 Cell Name   
LOT No.   021899 Lot Specification   distribution
Medium   Eagle's minimal essential medium(glutamine0.6g/L) 10% fetal calf serum Temperature   37 C
Cell Density at Seeding   2.0 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   ca 40hrs Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   94% Antibiotics Used   free
Passage Number   P16* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0625 Cell Name   Ca9-22
LOT No.   090898 Lot Specification   distribution
Medium   Minimal essential medium with 10% fetal calf serum(Mitsubishi PVF01). Temperature   37 C
Cell Density at Seeding   2 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.04% trypsin and 0.02% EDTA for 5 minutes at room temperature.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   98.8 Antibiotics Used   free
Passage Number   P13* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(type B), NP and LDH examined. human non-HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0625 Cell Name   Ca9-22
LOT No.   091985 Lot Specification   distribution
Medium   Eagle's MEM(glutamine 0.6g/L) + 10%FCS Temperature   37
Cell Density at Seeding   5x10^4cells/5ml Methods for Passages   0.04%trypsine/PBS, 1/20 - 1/30 dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P468 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0625 Cell Name   Ca9-22
LOT No.   11182016 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   0.5 - 1.0 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.04% trypsin and 0.02% EDTA in PBS.
Doubling Time   approx. 20 hrs. Cell Number in Vial (cells/1ml)   3.0 x 10^6
Viability at cell freezing (%)   93.8 Antibiotics Used   free
Passage Number   Unknown (16 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   93.8 Additional information   
Cell No.   JCRB0625 Cell Name   Ca9-22
LOT No.   03112021 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sgma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   3.3 - 9.8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.04% trypsin and 0.02% EDTA.
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   100 Antibiotics Used   free
Passage Number   Unknown (16 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   97 Additional information   
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