JCRB0701 FM3A
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0701 | Cell Name | FM3A |
|---|---|---|---|
| Profile | mammary carcinoma, spontaneously obtained, (C3H/He mouse) | Other Name | FM3A |
| Animal | mouse | Strain | C3H/He |
| Genus | Mus | Species | musclus |
| Sex | F | Age | |
| Identity | Tissue for Primary Cancer | mammary | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | mammary carcinoma, spontaneously obtained | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | mammary carcinoma, spontaneously obtained |
| Classify | Established by | Saito,H. & Nakano,N. | |
| Registered by | Umeda,M. | Regulation for Distribution | |
| Comment | High growth rate. PE. near 100 %. Easy to obtain mutant cell line. | Year | 1985 |
| Medium | ES medium({3051}) with 2% fetal calf serum or E.MEM with 10% fetal calf serum. | Methods for Passages | Prepare cell suspension by pipetting and dilute. |
| Cell Number on Passage | 1-2 x 10^4 cells/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | mammary gland |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:7105014 | Adenine phosphoribosyltransferase deficiency in cultured mouse mammary tumor FM3A cells resistant to 4-carbamoylimidazolium 5-olate. Koyama H,Kodama H Cancer Res. 1982 Oct;42(10):4210-4 |
| Pubmed id:5528803 | Initiation of a differentiated function (hyaluronic acid synthesis) by hybrid formation in culture. Koyama H,Ono T Biochim Biophys Acta. 1970 Oct 15;217(2):477-87 |
| Pubmed id:5956504 | Long-term culture of the ascites form of a mouse mammary tumor in serum-free media. Yamane I,Nakano N Tohoku J Exp Med. 1966 Mar 25;88(3):203-14 |
| Pubmed id:5920633 | Establishment of cell lines in vitro from a mammary ascites tumor of mouse and biological properties of the established lines in a serum containing medium. Nakano N Tohoku J Exp Med. 1966 Jan 25;88(1):69-84 |
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LOT Information
| Cell No. | JCRB0701 | Cell Name | FM3A |
|---|---|---|---|
| LOT No. | 043097 | Lot Specification | distribution |
| Medium | Eagle's minimal essential medium with 10% heat inactivated fetal calf serum (Mitsubishi PVF001). | Temperature | 37 C |
| Cell Density at Seeding | 2.1x10^4 cells/ml | Methods for Passages | Simple dilution. |
| Doubling Time | 10.6 hrs | Cell Number in Vial (cells/1ml) | 4.6x10^6 |
| Viability at cell freezing (%) | 89.0 | Antibiotics Used | free |
| Passage Number | P3* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | under examination |
| Chromosome Mode | NT | Chromosome Information | under examination |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO. | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB0701 | Cell Name | FM3A |
|---|---|---|---|
| LOT No. | 053095 | Lot Specification | FDSC/distribution |
| Medium | ES medium with 2% fetal bovine serum (JRH12103-78P). (ES medium=Nissui,031406) | Temperature | 37 C |
| Cell Density at Seeding | 5 x 10^4 cells/ml | Methods for Passages | Simple suspension by pipetting. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.78x10^6 |
| Viability at cell freezing (%) | 96.6 | Antibiotics Used | Km |
| Passage Number | P3* | PDL | NT |
| Sterility: MYCOPLASMA | NT | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0701 | Cell Name | FM3A |
|---|---|---|---|
| LOT No. | 092085 | Lot Specification | distribution |
| Medium | ES+2%FCS or MEM + 10%FCS | Temperature | 37 C |
| Cell Density at Seeding | 1-2 x 10^4 cells/ml | Methods for Passages | Prepare cell suspension by pipetting and dilute. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | |
| Viability at cell freezing (%) | NT | Antibiotics Used | |
| Passage Number | PDL | ||
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | Chromosome Information | NT | |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |