JCRB0704 FM3A TK~

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0704 Cell Name   FM3A TK~
Profile   mammary carcinoma, thymidine kinase-deficient FM3A, (C3H/He mouse) Other Name   FM3A TK~
Animal   mouse Strain   C3H/He
Genus   Mus Species   musclus
Sex   F Age   
Identity    Tissue for Primary Cancer   mammary
Case history    Metastasis   
Tissue Metastasized    Genetics   mammary carcinoma, thymidine kinase deficient.
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   mammary carcinoma
Classify   mutant Established by   Ayusawa,D.
Registered by   Umeda,M. Regulation for Distribution   
Comment    Year   1985
Medium   ES medium ({3051} ) with 2% fetal calf serum or E.MEM with 10% calf serum or fetal calf serum. Methods for Passages   Prepare cell suspension by pipetting.
Cell Number on Passage   2-3 x 10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   mammary gland
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J370
Reference
Pubmed id:7105014Adenine phosphoribosyltransferase deficiency in cultured mouse mammary tumor FM3A cells resistant to 4-carbamoylimidazolium 5-olate.
Koyama H,Kodama H
Cancer Res. 1982 Oct;42(10):4210-4
Pubmed id:208186A mammalian cell mutant with temperature-sensitive thymidine kinase.
Nakano MM,Sekiguchi T,Yamada MA
Somatic Cell Genet. 1978 Mar;4(2):169-78
Pubmed id:5920633Establishment of cell lines in vitro from a mammary ascites tumor of mouse and biological properties of the established lines in a serum containing medium.
Nakano N
Tohoku J Exp Med. 1966 Jan 25;88(1):69-84

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LOT Information

Cell No.   JCRB0704 Cell Name   FM3A TK~
LOT No.   012197 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   G6PD, LDH, NP. (mouse)
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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