JCRB0706 tsFT101

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0706 Cell Name   tsFT101
Profile   FM3A derivative, cytokinesis is blocked at 39 C, (C3H mouse) Other Name   28A9
Animal   mouse Strain   C3H
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   mammary
Case history    Metastasis   
Tissue Metastasized    Genetics   FM3A derivative
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Cytokinesis is blocked at 39 C.
Classify   mutant Established by   Yamada,M.
Registered by   Hanaoka,F. Regulation for Distribution   Require the permission of the originator.
Comment   Nucleus separation is performed while cytokinesis is blocked at non permissive temp. (39 C) resulting a poly nucleated cells. Year   5
Medium   RPMI1640 medium with 10% fetal calf serum. Methods for Passages   Pipetting gently to make single cell suspension, followed by changing medium.
Cell Number on Passage   5 x 10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   hybridoma
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J378
Reference
Pubmed id:2581712Isolation and initial characterization of a temperature-sensitive mutant of mouse FM3A cells defective in cytokinesis.
Yasumitsu H,Hanaoka F,Yasuda H,Murakami Y,Enomoto T,Yamada M
Cell Struct Funct. 1985 Mar;10(1):79-88

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LOT Information

Cell No.   JCRB0706 Cell Name   tsFT101
LOT No.   042997 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat inactivated calf serum (Hyclone 2111499). Temperature   37 C
Cell Density at Seeding   5x10^5 cells/ml Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.4x10^6
Viability at cell freezing (%)   90.0 Antibiotics Used   free
Passage Number   P4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB0706 Cell Name   tsFT101
LOT No.   102185 Lot Specification   distribution
Medium   RPMI1640 + CS 10 % Temperature   33
Cell Density at Seeding   5 x 10^4/ml Methods for Passages   Pipetting gently to make single cell suspension, followed by changing medium.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2x10^6/ml
Viability at cell freezing (%)   NT Antibiotics Used   
Passage Number   uk PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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