JCRB0707 V79 HPRT~

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0707 Cell Name   V79 HPRT~
Profile   V79 derivative, HPRT-deficient Other Name   T2-14
Animal   hamster, Chinese Strain   Chinese
Genus   Cricetulus Species   griseus
Sex   M Age   
Identity    Tissue for Primary Cancer   lung
Case history    Metastasis   
Tissue Metastasized    Genetics   lung (M)
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   HPRT~ cells.
Classify   mutant Established by   Ochi,T.
Registered by   Umeda,M. Regulation for Distribution   
Comment   Mycoplasma was eliminated by antibiotics. Clone D-7 was taken by limiting dilution method. 6TG-r, HAT-s was confirmed. Year   1985
Medium   Eagle's minimal essential medium with 10% fetal calf serum. Methods for Passages   0.05 % trypsin
Cell Number on Passage   2-3x10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8556
Pubmed id:6935177Mutagenesis assays with mammalian cells.
Kuroki T,Abbondandolo A,Drevon C,Huberman E,Laval F
IARC Monogr Eval Carcinog Risk Chem Hum Suppl. 1980;(2 Suppl):107-33
Pubmed id:4361348Colony formation of mammalian cells on agar plates and its application to Lederberg's replica plating.
Kuroki T
Exp Cell Res. 1973 Jul;80(1):55-62
Pubmed id:13901497The evaluation of x-ray-induced chromosome aberrations in cell-cultures of the Chinese hamster.
Int J Radiat Biol Relat Stud Phys Chem Med. 1961 Nov;4():185-210
Pubmed id:13726391Radiation response of mammalian cells grown in culture. 1. Repair of X-ray damage in surviving Chinese hamster cells.
Radiat Res. 1960 Oct;13():556-93
Pubmed id:13576097Observations on the chromosomes of Chinese hamster cells in tissue culture.
J Natl Cancer Inst. 1958 Aug;21(2):393-425


LOT Information

Cell No.   JCRB0707 Cell Name   
LOT No.   062498 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   2-3 x 10^4 cells/ml Methods for Passages   0.05% trypsin (0.25% trypsin and 0.02% EDTA was used in this lot)
Doubling Time    Cell Number in Vial (cells/1ml)   2.7 x 10^6
Viability at cell freezing (%)   90.0 Antibiotics Used   free
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as Chinese hamster by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information