JCRB0711 T24

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0711 Cell Name   T24
Profile   urinary bladder carcinoma Other Name   T24
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   81
Identity   available Tissue for Primary Cancer   bladder
Case history    Metastasis   
Tissue Metastasized    Genetics   urinary bladder carcinoma
Life Span    Crisis PDL   
Morphology    Character   urinary bladder carcinoma
Classify    Established by   Bubenik,J. et al.
Registered by   Umeda,M. Regulation for Distribution   
Comment   Ha-ras mutation Year   1985
Medium   Eagle's minimal essential medium with 10% fetal calf serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   10^5 cells/60mm dish Race   
CO2 Conc.   5 % Tissue Sampling   bladder
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0554
Reference
Pubmed id:6823318 Identity of some human bladder cancer cell lines.
O'Toole CM,Povey S,Hepburn P,Franks LM
Nature. 1983 Feb 3;301(5899):429-30
Pubmed id:6298635 Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologue.
Capon DJ,Chen EY,Levinson AD,Seeburg PH,Goeddel DV
Nature. 1983 Mar 3;302(5903):33-7
Pubmed id:864752 Markers of neoplastic transformation in epithelial cell lines derived from human carcinomas.
Marshall CJ,Franks LM,Carbonell AW
J Natl Cancer Inst. 1977 Jun;58(6):1743-51
Pubmed id:4133950 Established cell line of urinary bladder carcinoma (T24) containing tumour-specific antigen.
Bubeník J,Baresová M,Viklický V,Jakoubková J,Sainerová H,Donner J
Int J Cancer. 1973 May;11(3):765-73

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LOT Information

Cell No.   JCRB0711 Cell Name   T24
LOT No.   101696 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0711 Cell Name   T24
LOT No.   061995 Lot Specification   FDSC/distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (JRH12103-78P) Temperature   37 C
Cell Density at Seeding   4.2x10^4 cells/ml Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.88x10^6
Viability at cell freezing (%)   94.2 Antibiotics Used   free
Passage Number   P3* PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0711 Cell Name   T24
LOT No.   072586 Lot Specification   distribution
Medium   MEM + 10%FCS Temperature   37
Cell Density at Seeding   10^5/60D Methods for Passages   trypsin
Doubling Time   NT Cell Number in Vial (cells/1ml)   5x10^5/ml
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P43 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0711 Cell Name   T24
LOT No.   11182014 Lot Specification   distribution
Medium   MEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   1.21-1.62x10^4cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.26x10^6
Viability at cell freezing (%)   96.8 Antibiotics Used   free
Passage Number   p43 PDL   NT
Sterility: MYCOPLASMA   -- Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,12
D13S317:12
D7S820:10,11
D16S539:9
VWA:17
TH01:6
AM:X
TPOX:8,11
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0711 Cell Name   T24
LOT No.   02052018 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   6 - 7 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   3.3 x 10^6
Viability at cell freezing (%)   84.1 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   84.1
Additional information   
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