JCRB0713 HeLa S3 (sc)

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0713 Cell Name   HeLa S3 (sc)
Profile   HeLa derivative, can grow in suspension culture Other Name   HeLa S3
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   31
Identity   available Tissue for Primary Cancer   uterine cervix
Case history    Metastasis   
Tissue Metastasized    Genetics   cervix, can grow in suspension culture
Life Span    Crisis PDL   
Morphology    Character   cervix, can grow in suspension culture
Classify    Established by   Gey,G.O.
Registered by   Umeda,M. Regulation for Distribution   
Comment   medium + 0.1 % pluronic F68, spiner culture applicable. Year   1985
Medium   Eagle's minimal essential medium with 10% calf serum Methods for Passages   Cells are treated with 0.05 % trypsin
Cell Number on Passage   5 x10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   cervix
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 +
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2934
Reference
Pubmed id:9892199Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping.
Macville M,Schröck E,Padilla-Nash H,Keck C,Ghadimi BM,Zimonjic D,Popescu N,Ried T
Cancer Res. 1999 Jan 1;59(1):141-50
Pubmed id:4361348Colony formation of mammalian cells on agar plates and its application to Lederberg's replica plating.
Kuroki T
Exp Cell Res. 1973 Jul;80(1):55-62
Pubmed id:5543947Localization of the replication point of mammalian cell DNA at the membrane.
Hanaoka F,Yamada M
Biochem Biophys Res Commun. 1971 Feb 19;42(4):647-53
Pubmed id:13669339Variation in plaque-forming ability among parental and clonal strains of HeLa cells.
DARNELL JE Jr,SAWYER TK
Virology. 1959 Jun;8(2):223-9
Pubmed id:17811344Molecular Growth Requirements of Single Mammalian Cells.
Sato G,Fisher HW,Puck TT
Science. 1957 Nov 8;126(3280):961-4
Pubmed id:13286432Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer.
PUCK TT,MARCUS PI,CIECIURA SJ
J Exp Med. 1956 Feb 1;103(2):273-83
Pubmed id:16589695A RAPID METHOD FOR VIABLE CELL TITRATION AND CLONE PRODUCTION WITH HELA CELLS IN TISSUE CULTURE: THE USE OF X-IRRADIATED CELLS TO SUPPLY CONDITIONING FACTORS.
Puck TT,Marcus PI
Proc Natl Acad Sci U S A. 1955 Jul 15;41(7):432-7

Images



LOT Information

Cell No.   JCRB0713 Cell Name   HeLa S3 (sc)
LOT No.   12072001 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   2-10 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time    Cell Number in Vial (cells/1ml)   3.2 x 10^6
Viability at cell freezing (%)   93.2 Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed by NP, G6PD (type A), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-EMEM CO2 Conc.   
Viability immediately after thawing (%)   95% Additional information   
Cell No.   JCRB0713 Cell Name   
LOT No.   04162001 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% calf serum Temperature   37 C
Cell Density at Seeding    Methods for Passages   0.05% trypsin
Doubling Time    Cell Number in Vial (cells/1ml)   5.8 x 10^6
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0713 Cell Name   
LOT No.   090095 Lot Specification   distribution
Medium   MEM + 10 % CS Temperature   37
Cell Density at Seeding    Methods for Passages   0.05 % trypsin
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number   unknown PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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