JCRB0717 MDCK.P3

Cell information

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無血清馴化株MDCK.P3について

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0717 Cell Name   MDCK.P3
Profile   normal kidney, MDCK derivative, no serum required Other Name   MDCK･P3
Animal   dog Strain   
Genus   Canis Species   familiaris
Sex    Age   
Identity    Tissue for Primary Cancer   kidney
Case history    Metastasis   
Tissue Metastasized    Genetics   kidney, no serum required
Life Span    Crisis PDL   
Morphology    Character   kidney, no serum required
Classify    Established by   Katsuta,H.
Registered by   Umeda,M. Regulation for Distribution   
Comment    Year   
Medium   DM160 medium. Methods for Passages   harvest cells by scraper and suspend in fresh medium
Cell Number on Passage   5x10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2586
Reference
Pubmed id:15234806Comparative study of influenza virus replication in Vero and MDCK cell lines.
Youil R,Su Q,Toner TJ,Szymkowiak C,Kwan WS,Rubin B,Petrukhin L,Kiseleva I,Shaw AR,DiStefano D
J Virol Methods. 2004 Sep 1;120(1):23-31
Pubmed id:212055A cell strain cultured from porcine kidney increases cyclic AMP content upon exposure to calcitonin or vasopressin.
Goldring SR,Dayer JM,Ausiello DA,Krane SM
Biochem Biophys Res Commun. 1978 Jul 28;83(2):434-40
Pubmed id:567227Polarized monolayers formed by epithelial cells on a permeable and translucent support.
Cereijido M,Robbins ES,Dolan WJ,Rotunno CA,Sabatini DD
J Cell Biol. 1978 Jun;77(3):853-80
Pubmed id:828141The origin and characteristics of a pig kidney cell strain, LLC-PK.
Hull RN,Cherry WR,Weaver GW
In Vitro. 1976 Oct;12(10):670-7

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LOT Information

Cell No.   JCRB0717 Cell Name   MDCK.P3
LOT No.   04302015 Lot Specification   distribution
Medium   DM-201 medium (serum-free) (株)機能性ペプチド研究所・特注 Temperature   37 C
Cell Density at Seeding   uncountable Methods for Passages   Scraping or the use of thermosensitive dish (RepCell)
Doubling Time    Cell Number in Vial (cells/1ml)   Uncountable
Viability at cell freezing (%)   Uncountabl Antibiotics Used   free
Passage Number   Unknown (4 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as dog by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   Cellbanker 2 CO2 Conc.   5%
Viability immediately after thawing (%)   uncountable Additional information   
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