JCRB0721 F9

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0721 Cell Name   F9
Profile   teratocarcinoma, differentiation-inducible, (129/Sv mouse) Other Name   F9
Animal   mouse Strain   129/SV
Genus   Mus Species   musclus
Sex   M Age   
Identity    Tissue for Primary Cancer   teratoma
Case history    Metastasis   
Tissue Metastasized    Genetics   teratocarcinoma
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   teratocarcinoma
Classify    Established by   Stevens,L.C.
Registered by   Umeda,M. Regulation for Distribution   
Comment   Mycoplasma was eliminated and clone B12 was isolated by limiting dilution method. Vitamin A was effective for the induction of development. Year   1986
Medium   Dulbecco's modified Eagle's medium with10 % fetal bovine serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   1-2x10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   testis (transplanted with embryo)
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0259
Reference
Pubmed id:6250719 Hormonal induction of differentiation in teratocarcinoma stem cells: generation of parietal endoderm by retinoic acid and dibutyryl cAMP.
Strickland S,Smith KK,Marotti KR
Cell. 1980 Sep;21(2):347-55
Pubmed id:6931103 Characterization of a human ovarian teratocarcinoma-derived cell line.
Zeuthen J,Nørgaard JO,Avner P,Fellous M,Wartiovaara J,Vaheri A,Rosén A,Giovanella BC
Int J Cancer. 1980 Jan 15;25(1):19-32
Pubmed id:214238 The induction of differentiation in teratocarcinoma stem cells by retinoic acid.
Strickland S,Mahdavi V
Cell. 1978 Oct;15(2):393-403
Pubmed id:1103673 [Mouse teratocarcinoma: differentiation in cultures of a multipotential primitive cell line (author's transl)].
Nicolas JF,Dubois P,Jakob H,Gaillard J,Jacob F
Ann Microbiol (Paris). 1975 Jan;126(1):3-22
Pubmed id:4355379 Surface antigens common to mouse cleavage embryos and primitive teratocarcinoma cells in culture.
Artzt K,Dubois P,Bennett D,Condamine H,Babinet C,Jacob F
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2988-92
Pubmed id:4521215 Alkaline phosphatase activity in mouse teratoma.
Berstine EG,Hooper ML,Grandchamp S,Ephrussi B
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3899-903

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LOT Information

Cell No.   JCRB0721 Cell Name   F9
LOT No.   110696 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (JRS D010400). Temperature   37 C
Cell Density at Seeding   2-3x10^4 cells/sq.cm. Methods for Passages   Treat cells with 0.05% trypsin and 0.2% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   6.1x10^6
Viability at cell freezing (%)   89.5 Antibiotics Used   free
Passage Number   P4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB0721 Cell Name   F9
LOT No.   041695 Lot Specification   FDSC/distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (Bio cell, 4001776) Temperature   37 C
Cell Density at Seeding   5.5x10^5 cells/ml Methods for Passages   Cells are treated with 0.125% trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   8.28x10^6
Viability at cell freezing (%)   89.9 Antibiotics Used   free
Passage Number   P3* PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0721 Cell Name   
LOT No.   041795 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (Bio cell, 4001776) Temperature   37 C
Cell Density at Seeding   5.5x10^5 cells/ml Methods for Passages   Cells are treated with 0.125% trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P3* PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0721 Cell Name   F9
LOT No.   07312017 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   3.0 - 8.8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 19 hrs. Cell Number in Vial (cells/1ml)   3.8 x 10^6
Viability at cell freezing (%)   88.5 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   88.5
Additional information   
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