JCRB0727 A9(An4)-2

Cell information

Important Notice(s)

ヒト単染色体導入ハイブリッド細胞の分譲について:鳥取大とのMTA締結が必要です

About the distribution of human monochromosomal hybrid cells: Requirement of MTA between customers and Tottori University

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0727 Cell Name   A9(An4)-2
Profile   contains human chromosome # 1 (MTA with Tottori Univ. is required.) Other Name   
Animal   human chromosome-introduced Strain   
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   human chromosomal hybrid
Case history    Metastasis   
Tissue Metastasized    Genetics   contains human chromosome #1
Life Span    Crisis PDL   
Morphology    Character   contains human chromosome #1
Classify    Established by   Oshimura,M.
Registered by   M. Oshimura Regulation for Distribution   MTA with Tottori Univ. is required.
Comment    Year   1988
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum and 800 ug/ml G418. Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA for 3 min. at 37 C.
Cell Number on Passage   1-2x10^4 cells/sq.cm. Race   
CO2 Conc.   5 % Tissue Sampling   hybrid
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2830
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LOT Information

Cell No.   JCRB0727 Cell Name   A9(An4)-2
LOT No.   090496 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum and 800 ug/ml G418 Temperature   37 C
Cell Density at Seeding   1-2 x 10^4 cells/sq.cm Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   87.0 Antibiotics Used   (G418)
Passage Number   P3* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse.
Chromosome Mode    Chromosome Information   
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
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