JCRB0733 PC-12

Cell information

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JCRB0733 PC-12の接着性について

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0733 Cell Name   PC-12
Profile   pheochromocytoma, , neuronal differentiation by NGF, adhesive variant Other Name   PC12
Animal   rat Strain   
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   neural
Case history   pheochromocytoma of the adrenal medulla. Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology    Character   Cells are differentiated into sympathetic neuron-like cells, when exposed to nerve growth factor.
Classify    Established by   Greene,L.
Registered by   T. Amano Regulation for Distribution   
Comment   relating to research for NGF Year   1988
Medium   RPMI1640 medium with 10 % horse serum and 5 % fetal bovine serum Methods for Passages   Mechanical dispersion by pipetting.
Cell Number on Passage   dilute to 70-80 % Race   
CO2 Conc.   5 % Tissue Sampling   chromaphin cell
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0481
Reference
Pubmed id:1493541Different ability in PC12 clones to recover from MMC toxicity following NGF treatment.
Iizuka H,Umeda M
Brain Res. 1992 Dec 18;599(1):1-5
Pubmed id:3839317Molecular cloning of a gene sequence regulated by nerve growth factor.
Levi A,Eldridge JD,Paterson BM
Science. 1985 Jul 26;229(4711):393-5
Pubmed id:6641712A macromolecular structure favouring microtubule assembly in NGF-differentiated pheochromocytoma cells (PC12).
Biocca S,Cattaneo A,Calissano P
EMBO J. 1983;2(5):643-8
Pubmed id:742018[Therapeutic results in adult acute leukemia in Giessen].
Löffler H,Pralle H,Graubner M,Schrezenmaier E
Verh Dtsch Ges Inn Med. 1978;(84):156-8
Pubmed id:1065897Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.
Greene LA,Tischler AS
Proc Natl Acad Sci U S A. 1976 Jul;73(7):2424-8

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LOT Information

Cell No.   JCRB0733 Cell Name   PC-12
LOT No.   06112010 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% horse serum and 5% fetal bovine serum Temperature   37 C
Cell Density at Seeding   1.2 x 1.9 x 10^5 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 33 hrs Cell Number in Vial (cells/1ml)   2.7 x 10^6
Viability at cell freezing (%)   97.0 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   Expected viability (defrost ampoule) 97 %.
Cell No.   JCRB0733 Cell Name   
LOT No.   10152007 Lot Specification   distribution
Medium   RPMI1640 medium with 10 % horse serum and 5 % fetal bovine serum (lot:GIBCO484375) Temperature   37C
Cell Density at Seeding   1.0x 10^5cells/ml Methods for Passages   pipetting
Doubling Time   25hr Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   P11＊ PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as rat by G6PD, NP, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yea Exoteric Gene   
Medium for Freezing   70% Culture medium + 20% FCS + 10% DMSO CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0733 Cell Name   
LOT No.   10222004 Lot Specification   distribution
Medium   RPMI1640 medium with 10% horse serum and 5% fetal bovine serum Temperature   37 C
Cell Density at Seeding   2 - 4 x 10^5 cells/ml Methods for Passages   Pipetting
Doubling Time   31 hrs Cell Number in Vial (cells/1ml)   3.1 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   unknown (P8 in bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0733 Cell Name   
LOT No.   05252000 Lot Specification   distribution
Medium   RPMI1640 medium with 10% horse serum and 5% fetal calf serum Temperature   37 C
Cell Density at Seeding   around 5 x 10^4 cells/ml Methods for Passages   mechanical dispersion by pipetting (use of trypsin is also acceptable)
Doubling Time   ca 34 hrs Cell Number in Vial (cells/1ml)   8.0 x 10^5
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   P8* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium with 10% DMSO and 20% serum CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0733 Cell Name   PC-12
LOT No.   083096 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0733 Cell Name   
LOT No.   012998 Lot Specification   distribution
Medium   RPMI1640 medium with 10% horse serum (heat-inacti-vation is recommended) and 5% fetal bovine serum Temperature   37 C
Cell Density at Seeding   5 x 10^4 cells/ml Methods for Passages   Mechanical dispersion by pipetting.
Doubling Time    Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   90.0 Antibiotics Used   free
Passage Number   P7* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0733 Cell Name   PC-12
LOT No.   06082017 Lot Specification   distribution
Medium   RPMI1640 medium with 10% horse serum and 5% fetal bovine serum Temperature   37 C
Cell Density at Seeding   approx. 3 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA (or mechanical dispersion).
Doubling Time   approx. 29 hrs. Cell Number in Vial (cells/1ml)   3.5 x 10^6
Viability at cell freezing (%)   80.9 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   10% DMSO, 20% serum - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   80.9 Additional information   
Cell No.   JCRB0733 Cell Name   PC-12
LOT No.   02212019 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% heat-inactivated horse serum and 5% fetal bovine serum Temperature   37 C
Cell Density at Seeding   1.5 - 2.9 x 10^5 cells/mL Methods for Passages   Cells were detached by pipetting, and were suspended to fresh medium.
Doubling Time   approx. 37 hrs. Cell Number in Vial (cells/1ml)   3.8 x 10^6
Viability at cell freezing (%)   82.8 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   83.1 Additional information   Some lots of horse serum may not support the cell attachment.
Cell No.   JCRB0733 Cell Name   PC-12
LOT No.   08072020 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% heat-inactivated horse serum (GIBCO Cat # 26050) and 5% fetal bovine serum (Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 1.5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Some cells will pile up and detach during culture.
Doubling Time   approx. 26 hrs. Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   80 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   Culture medium with 10% DMSO CO2 Conc.   5%
Viability immediately after thawing (%)   77 Additional information   
Cell No.   JCRB0733 Cell Name   PC-12
LOT No.   01172022 Lot Specification   distribution
Medium    RPMI 1640 medium with 10% heat-inactivated horse serum (GIBCO Cat # 10050) and 5% fetal bovine serum (Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.2 - 1.5 x 10^5 cells/mL Methods for Passages   Mechanical dispersion by pipetting.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   77.3 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   5% DMSO - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   82.4 Additional information   Some cells will pile up and form cell aggregates.
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