JCRB0734 3Y1-B Clone 1-6

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0734 Cell Name   3Y1-B Clone 1-6
Profile   fibroblast, whole embryo, (Fischer 344 rat) Other Name   3Y1
Animal   rat Strain   Fischer 344
Genus   Rattus Species   norvegicus
Sex   F Age   
Identity    Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   Ref{4007}showed this cell line is mixed population
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   whole embryo
Classify   new Established by   Kimura,G.
Registered by    Regulation for Distribution   Citation is required when published. #627.
Comment    Year   1988
Medium   Dulbecco's modified Eagle's medium with 0.1% glucose and 10% fetal calf serum Methods for Passages   0.25%trypsin. Dilute 1/20. Prepare many frozen ampules when received. Use new stock after 1-2 mo.cul
Cell Number on Passage   Less than 5-8x10^5 cells/50mm Race   
CO2 Conc.   5 % Tissue Sampling   embryo/fetus
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_E147
Reference
Pubmed id:8014101 Presence of p53 mutations in 3Y1-B clone 1-6: a rat cell line widely used as a normal immortalized fibroblast.
Ushijima T,Makino H,Nakayasu M,Aonuma S,Takeuchi M,Segawa K,Sugimura T,Nagao M
Jpn J Cancer Res. 1994 May;85(5):455-8
Pubmed id:2553277 Functional rescue of temperature-sensitive defects in the cell-cycle mutants of rat 3Y1 fibroblasts by the small t-antigen deletion mutant of simian virus 40.
Matsuzaki A,Ohno K,Ohtsu M,Kimura G
Cell Struct Funct. 1989 Aug;14(4):375-82
Pubmed id:166944 Rat cell line 3y1 and its virogenic polyoma- and sv40- transformed derivatives.
Kimura G,Itagaki A,Summers J
Int J Cancer. 1975 Apr 15;15(4):694-706
Pubmed id:4301647 Basis for the acquisition of malignant potential by mouse cells cultivated in vitro.
Aaronson SA,Todaro GJ
Science. 1968 Nov 29;162(3857):1024-6

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LOT Information

Cell No.   JCRB0734 Cell Name   3Y1-B Clone 1-6
LOT No.   050295 Lot Specification   FDSC/distribution
Medium   Dulbecco's modified Eagle's medium with 0.1% glucose and 10% fetal bovine serum (Bio cell, 4001776) Temperature   37 C
Cell Density at Seeding   1.97x10^4 cells/ml Methods for Passages   Cells are treated with 0.125% and 0.02% EDTA and cells are diluted to 1/20.
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.6x10^5
Viability at cell freezing (%)   98.1 Antibiotics Used   free
Passage Number   P6* PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0734 Cell Name   3Y1-B Clone 1-6
LOT No.   03172017 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (low glucose) and 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   4.5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1 day. Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   94.9 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   94.9
Additional information   
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