JCRB0735 SV-3Y1-C66

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0735 Cell Name   SV-3Y1-C66
Profile   3Y1 derivative, SV40-transformed, (Fischer rat) Other Name   
Animal   rat Strain   Fischer
Genus   Rattus Species   norvegicus
Sex   F Age   
Identity    Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   whole embryo, SV40 transformed subline of 3Y1
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   SV40 transformed subline of 3Y1
Classify   transformed Established by   Kimura,G.
Registered by   Kimura,G. Regulation for Distribution   Citation of #966 is required when published.
Comment    Year   1988
Medium   Dulbecco's modified Eagle's medium with 0.1% glucose and 10% fetal calf serum. Methods for Passages   Cells harvested after treatment with 0.01% trypsin and 0.02% EDTA. Dilute 1/20-1/40 when subculture.
Cell Number on Passage   8.5 x 10^3 cells/sq.cm. Race   
CO2 Conc.   5 % Tissue Sampling   embryo/fetus whole body
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_F822
Reference
Pubmed id:2550745Frequency of cell transformation by the small DNA tumor viruses: infection of proliferating cells and quiescent cells.
Matsuzaki A,Okuda A,Tamura H,Ohtsu M,Kimura G
Microbiol Immunol. 1989;33(8):657-67
Pubmed id:2844831Influence of the deprivation of a single amino acid on cellular proliferation and survival in rat 3Y1 fibroblasts and their derivatives transformed by a wide variety of agents.
Tanaka H,Zaitsu H,Onodera K,Kimura G
J Cell Physiol. 1988 Sep;136(3):421-30
Pubmed id:680001A temperature-sensitive mammalian cell mutant exhibiting micronucleation.
Sato K,Hama-Inaba H
Exp Cell Res. 1978 Jul;114(2):484-6

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LOT Information

Cell No.   JCRB0735 Cell Name   
LOT No.   04212003 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (lot; BioWhittaker 0S069F) Temperature   37 C
Cell Density at Seeding   1:10-1:20 split Methods for Passages   0.05% trypsin and 0.2% EDTA
Doubling Time   16 hrs Cell Number in Vial (cells/1ml)   3.9 x 10^6
Viability at cell freezing (%)   91.8 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
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