JCRB0738 E1A-3Y1-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0738 Cell Name   E1A-3Y1-1
Profile   3Y1 derivative, transformed by E1A of Ad12 virus, (Fischer rat) Other Name   
Animal   rat Strain   Fischer
Genus   Rattus Species   norvegicus
Sex   F Age   
Identity    Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   whole embryo, transformed 3Y1 by E1A of Ad12 virus
Life Span    Crisis PDL   
Morphology    Character   whole embryo, transformed 3Y1 by E1A of Ad12 virus
Classify   new Established by   Kimura,G.
Registered by    Regulation for Distribution   Citation of #967 is required.
Comment    Year   1988
Medium   Dulbecco's modified Eagle's medium(0.1% glucose) with 10% fetal calf serum. Methods for Passages   0.05 % trypsin+5mM EDTA. Dilute 1/20-1/40. Prepare many ampules when received. Use new ampule after 1-2 month culture.
Cell Number on Passage   Below 1x10^6 cells/50mm dish. Race   
CO2 Conc.   5 % Tissue Sampling   embryo/fetus whole
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_H226
Reference
Pubmed id:2920374The high sensitivity of cells transformed by E1A gene of adenovirus type 12 to diacylglycerol-mediated cell killing.
Shimura H,Matsuzaki A,Shiroki K,Ohtsu M,Fujinaga K,Onodera K,Kimura G
Cancer Lett. 1989 Feb;44(2):143-9
Pubmed id:2844831Influence of the deprivation of a single amino acid on cellular proliferation and survival in rat 3Y1 fibroblasts and their derivatives transformed by a wide variety of agents.
Tanaka H,Zaitsu H,Onodera K,Kimura G
J Cell Physiol. 1988 Sep;136(3):421-30
Pubmed id:2961438Selective cytotoxicity of phospholipids and diacylglycerols to rat 3Y1 fibroblasts transformed by adenovirus type 12 or its E1A gene.
Shimura H,Ohtsu M,Matsuzaki A,Mitsudomi T,Onodera K,Kimura G
Cancer Res. 1988 Feb 1;48(3):578-83

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LOT Information

Cell No.   JCRB0738 Cell Name   
LOT No.   02132006 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS Lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   3 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   3.1 x 10^6
Viability at cell freezing (%)   98.2 Antibiotics Used   free
Passage Number   P12 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
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