JCRB0742 SR-3Y1-2
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0742 | Cell Name | SR-3Y1-2 |
|---|---|---|---|
| Profile | 3Y1 derivative, transformed by Rous sarcoma virus, (Fischer rat) | Other Name | |
| Animal | rat | Strain | Fischer |
| Genus | Rattus | Species | norvegicus |
| Sex | F | Age | |
| Identity | Tissue for Primary Cancer | embryo | |
| Case history | 3Y1 transformed by virus | Metastasis | |
| Tissue Metastasized | Genetics | whole embryo,transformed 3Y1 by Rous sarcoma virus | |
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | whole embryo,transformed 3Y1 by Rous sarcoma virus |
| Classify | transformed | Established by | Kimura,G. |
| Registered by | Kimura,G. | Regulation for Distribution | Citation of #966 is required. |
| Comment | Year | 1988 | |
| Medium | Dulbecco's modified Eagle's medium with 0.1% glucose and 10% fetal calf serum. | Methods for Passages | Cells treated with 0.05% trypsin and 5mM EDTA when subculture. Dilution 1/10. |
| Cell Number on Passage | Below 1x10^6 cells/50mm dish. | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | embryo/fetus whole |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:2844831 | Influence of the deprivation of a single amino acid on cellular proliferation and survival in rat 3Y1 fibroblasts and their derivatives transformed by a wide variety of agents. Tanaka H,Zaitsu H,Onodera K,Kimura G J Cell Physiol. 1988 Sep;136(3):421-30 |
| Pubmed id:680001 | A temperature-sensitive mammalian cell mutant exhibiting micronucleation. Sato K,Hama-Inaba H Exp Cell Res. 1978 Jul;114(2):484-6 |
| Images |
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LOT Information
| Cell No. | JCRB0742 | Cell Name | |
|---|---|---|---|
| LOT No. | 092299 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) | Temperature | 37 C |
| Cell Density at Seeding | 2 - 5 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Doubling Time | D.T. = 22 - 25 hrs | Cell Number in Vial (cells/1ml) | 1.1 x 10^6 |
| Viability at cell freezing (%) | 91 | Antibiotics Used | free |
| Passage Number | P6* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as rat by NP, G6PD, AST. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |