JCRB0813 VMRC-RCW

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0813 Cell Name   VMRC-RCW
Profile   renal cell carcinoma Other Name   Thomas Wirts
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   
Identity   available Tissue for Primary Cancer   kidney
Case history    Metastasis   
Tissue Metastasized    Genetics   renal cell carcinoma
Life Span   infinite Crisis PDL   
Morphology    Character   renal cell carcinoma
Classify    Established by   Akiyama,M.
Registered by   Akiyama,M. Regulation for Distribution   
Comment    Year   1987
Medium   Eagle's minimal essential medium with 10% fetal calf serum Methods for Passages   0.25% trypsin and dilute in fresh medium. Change medium twice a week
Cell Number on Passage   split ratio=1/6 Race   
CO2 Conc.   5 % Tissue Sampling   renal
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1790
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1240224
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LOT Information

Cell No.   JCRB0813 Cell Name   
LOT No.   10052009 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 6 - 7 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 27 hrs Cell Number in Vial (cells/1ml)   8.6 x 10^5
Viability at cell freezing (%)   97.7 Antibiotics Used   free
Passage Number   P29 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0813 Cell Name   
LOT No.   090595 Lot Specification   distribution
Medium   MEM +10%FCS Temperature   37
Cell Density at Seeding    Methods for Passages   0.25% trypsin and dilute in fresh medium. Change medium twice a week.
Doubling Time    Cell Number in Vial (cells/1ml)   ca3-5x10^6
Viability at cell freezing (%)    Antibiotics Used   penicillin/streptomycin
Passage Number   P23 PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   confirmed as human
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0813 Cell Name   
LOT No.   102398 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T.=ca. 60 hrs Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   91.0 Antibiotics Used   free
Passage Number   P29 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type A), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0813 Cell Name   VMRC-RCW
LOT No.   11082016 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   2.8 - 6.0 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 35 hrs. Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   P29 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   93
Additional information   
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