-Detailed Information [JCRB0813]-

JCRB0813 VMRC-RCW

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0813	Cell Name	VMRC-RCW
Profile	renal cell carcinoma	Other Name	Thomas Wirts
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	M	Age
Identity	available	Tissue for Primary Cancer	kidney
Case history		Metastasis
Tissue Metastasized		Genetics	renal cell carcinoma
Life Span	infinite	Crisis PDL
Morphology		Character	renal cell carcinoma
Classify		Established by	Akiyama,M.
Registered by	Akiyama,M.	Regulation for Distribution
Comment		Year	1987
Medium	Eagle's minimal essential medium with 10% fetal calf serum	Methods for Passages	0.25% trypsin and dilute in fresh medium. Change medium twice a week
Cell Number on Passage	split ratio=1/6	Race
CO2 Conc.	5 %	Tissue Sampling	renal
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_1790
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	1240224

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LOT Information

10052009
090595
102398
11082016
07142023

Cell No.	JCRB0813	Cell Name
LOT No.	10052009	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	approx. 6 - 7 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	approx. 27 hrs	Cell Number in Vial (cells/1ml)	8.6 x 10^5
Viability at cell freezing (%)	97.7	Antibiotics Used	free
Passage Number	P29	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0813	Cell Name
LOT No.	090595	Lot Specification	distribution
Medium	MEM +10%FCS	Temperature	37
Cell Density at Seeding		Methods for Passages	0.25% trypsin and dilute in fresh medium. Change medium twice a week.
Doubling Time		Cell Number in Vial (cells/1ml)	ca3-5x10^6
Viability at cell freezing (%)		Antibiotics Used	penicillin/streptomycin
Passage Number	P23	PDL
Sterility: MYCOPLASMA		Sterility: BACTERIA
Sterility: FUNGI		Isozyme Analysis	confirmed as human
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0813	Cell Name
LOT No.	102398	Lot Specification	distribution
Medium	Eagle's minimal essential medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121)	Temperature	37 C
Cell Density at Seeding	10^5 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	D.T.=ca. 60 hrs	Cell Number in Vial (cells/1ml)	2.4 x 10^6
Viability at cell freezing (%)	91.0	Antibiotics Used	free
Passage Number	P29	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type A), LD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0813	Cell Name	VMRC-RCW
LOT No.	11082016	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	2.8 - 6.0 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 35 hrs.	Cell Number in Vial (cells/1ml)	1.6 x 10^6
Viability at cell freezing (%)	93	Antibiotics Used	free
Passage Number	P29	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	93	Additional information

Cell No.	JCRB0813	Cell Name	VMRC-RCW
LOT No.	07142023	Lot Specification	distribution
Medium	EMEM with 10% heat inactivated fetal bovine serum (SIGMA, Cat#172012, Lot#12J396)	Temperature	37 C
Cell Density at Seeding	1.48-1.95x10^4 cells/sq.cm	Methods for Passages	Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.62x10^6
Viability at cell freezing (%)	99	Antibiotics Used	free
Passage Number	p29	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:12 D13S317:9 D7S820:8,10 D16S539:11,15 VWA:14,15 TH01:6,9.3 AM:Y TPOX:8 CSF1PO:11,12
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD)	CO2 Conc.	5%
Viability immediately after thawing (%)	94	Additional information

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