-Detailed Information [JCRB0815]-

JCRB0815 ABC-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0815	Cell Name	ABC-1
Profile	adenocarcinoma	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	M	Age	47
Identity	available	Tissue for Primary Cancer	lung (cancer)
Case history		Metastasis
Tissue Metastasized		Genetics	adenocarcinoma
Life Span		Crisis PDL
Morphology		Character	adenocarcinoma
Classify		Established by	Hiraki,S.
Registered by	Akiyama,M.	Regulation for Distribution
Comment		Year	1987
Medium	Eagle's minimal essential medium with 10% fetal calf serum	Methods for Passages	0.25% trypsin and dilute in fresh medium. Change medium twice a week
Cell Number on Passage	split ratio=1/6	Race
CO2 Conc.	5 %	Tissue Sampling	lung
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_1066
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	906791

Reference
Pubmed id:1752082	The spread of human lung cancer cells on collagens and its inhibition by type III collagen. Hirai K,Shimada H,Ogawa T,Taji S Clin Exp Metastasis. 1991 Nov-Dec;9(6):517-27

Images

LOT Information

02282012
07052010
12222006
090695
092598

Cell No.	JCRB0815	Cell Name	ABC-1
LOT No.	02282012	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	1 - 2 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 52 hrs.	Cell Number in Vial (cells/1ml)	1.5 x 10^6
Viability at cell freezing (%)	91.4	Antibiotics Used	free
Passage Number	P40	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0815	Cell Name	ABC-1
LOT No.	07052010	Lot Specification	distribution
Medium	Eagle's minimum essential medium(Nissui) with 10% heat inactivated fetal bovine serum(SIGMA027K03911)	Temperature	37 C
Cell Density at Seeding	2.89-5.58x10^4cells/sq.cm	Methods for Passages	Cells were harvested after treatment with 0.25%trypsin(GIBCO) and dilute in fresh medium.Change medium twice a week .Split ratio=1/6
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.47x10^6
Viability at cell freezing (%)	90.8	Antibiotics Used	free
Passage Number	p39	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:12 D13S317:8 D7S820:10,12 D16S539:9,12 VWA:16 TH01:10 AM:X TPOX:8,11 CSF1PO:12
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Cell Banker BLC-1(Nihon Zenyaku Industries)	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0815	Cell Name
LOT No.	12222006	Lot Specification	distribution
Medium	Eagle's minimal essential medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S)	Temperature	37 C
Cell Density at Seeding	1 - 2 x 10^5 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	approx. 2 days	Cell Number in Vial (cells/1ml)	2.2 x 10^6
Viability at cell freezing (%)	98	Antibiotics Used	free
Passage Number	P39	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B)MD, LD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0815	Cell Name
LOT No.	090695	Lot Specification	distribution
Medium	MEM + 10%FCS	Temperature	37
Cell Density at Seeding		Methods for Passages	0.25% trypsin and dilute in fresh medium. Change medium twice a week.
Doubling Time		Cell Number in Vial (cells/1ml)	ca3-5x10^6
Viability at cell freezing (%)		Antibiotics Used	penicillin/streptomycin
Passage Number	P35 (BM3)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	confirmed as human
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0815	Cell Name
LOT No.	092598	Lot Specification	distribution
Medium	Eagle's minimal essential medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121)	Temperature	37 C
Cell Density at Seeding	2-4 x 10^5 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	53 hrs	Cell Number in Vial (cells/1ml)	4.4 x 10^6
Viability at cell freezing (%)	96.0	Antibiotics Used	free
Passage Number	P40	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD, AST.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information