JCRB0817 SBC-2
Cell information
Important Notice(s)JCRB0816 SBC-1細胞とJCRB0817 SBC-2細胞の由来について
JCRB0816 SBC-1 and JCRB0817 SBC-2: the problem of origin
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0817 | Cell Name | SBC-2 |
|---|---|---|---|
| Profile | Stop providing for the issue of quality control. (Please contact us for more information) small cell carcinoma, intermediate, JCRB0816 SBC-1 and JCRB0817 SBC-2 exhibit the same DNA profile. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | lung (cancer) |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | small cell carcinoma (intermediate) | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | small cell carcinoma (intermediate) |
| Classify | Established by | Hiraki,S. | |
| Registered by | Akiyama,M. | Regulation for Distribution | |
| Comment | Year | 1987 | |
| Medium | RPMI1640 medium with 15% fetal calf serum | Methods for Passages | |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | lung |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:1330284 | In vitro measurement of chemosensitivity of human small cell lung and gastric cancer cell lines toward cell cycle phase-nonspecific agents under the clinically equivalent area under the curve. Mitsuhashi Y,Inaba M,Sugiyama Y,Kobayashi T Cancer. 1992 Nov 15;70(10):2540-6 |
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LOT Information
| Cell No. | JCRB0817 | Cell Name | |
|---|---|---|---|
| LOT No. | 01212002 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% fetal calf serum | Temperature | 37 C |
| Cell Density at Seeding | 2-7 x 10^5 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA. (Easy to detach by pipetting without trypsin treatment) |
| Doubling Time | D.T. = ca 38 hrs | Cell Number in Vial (cells/1ml) | 5.0 x 10^6 |
| Viability at cell freezing (%) | 82 | Antibiotics Used | free |
| Passage Number | P46 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD, AST. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% FCS-RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0817 | Cell Name | |
|---|---|---|---|
| LOT No. | 081195 | Lot Specification | distribution |
| Medium | RPMI1640 + 15% FCS | Temperature | 37 |
| Cell Density at Seeding | split ratio=1:6 | Methods for Passages | dilution |
| Doubling Time | Cell Number in Vial (cells/1ml) | ca3-5x10^6 | |
| Viability at cell freezing (%) | Antibiotics Used | penicillin/streptomycin | |
| Passage Number | P53 (BM3) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | confirmed as human |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0817 | Cell Name | |
|---|---|---|---|
| LOT No. | 091895 | Lot Specification | distribution |
| Medium | RPMI1640 + 15% FCS | Temperature | 37 |
| Cell Density at Seeding | split ratio=1:6 | Methods for Passages | dilution |
| Doubling Time | Cell Number in Vial (cells/1ml) | ca3-5x10^6 | |
| Viability at cell freezing (%) | Antibiotics Used | penicillin/streptmycin | |
| Passage Number | P39 (BM3) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | confirmed as human |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |