JCRB0823 YMB-1
Cell information
Important Notice(s)JCRB0823 YMB-1, JCRB0825 YMB-1-Eについてのご注意
JCRB0823 YMB-1, JCRB0825 YMB-1-E: the problem of origin
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0823 | Cell Name | YMB-1 |
|---|---|---|---|
| Profile | breast cancer, exhibit the same DNA profile as other breast carcinoma cell line ZR-75-1. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 55 |
| Identity | available | Tissue for Primary Cancer | mammary |
| Case history | Metastasis | No | |
| Tissue Metastasized | Genetics | breast cancer | |
| Life Span | Crisis PDL | ||
| Morphology | Character | breast cancer | |
| Classify | Established by | Yamane,M. | |
| Registered by | Akiyama,M. | Regulation for Distribution | |
| Comment | Year | 1987 | |
| Medium | RPMI1640 medium with 10% fetal calf serum | Methods for Passages | Cells are loosely adherent, and attachment is poor. Cells are treated with 0.25% trypsin and dilute in fresh medium. Change medium twice a week |
| Cell Number on Passage | split ratio=1/6 | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | breast |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:6534930 | Establishment and characterization of new cell line (YMB-1) derived from human breast carcinoma. Yamane M,Nishiki M,Kataoka T,Kishi N,Amano K,Nakagawa K,Okumichi T,Naito M,Ito A,Ezaki H Hiroshima J Med Sci. 1984 Dec;33(4):715-20 |
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LOT Information
| Cell No. | JCRB0823 | Cell Name | |
|---|---|---|---|
| LOT No. | 10202004 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) | Temperature | 37 C |
| Cell Density at Seeding | 1 - 2 x 10^5 cells/ml | Methods for Passages | pipetting |
| Doubling Time | approx.48 hrs | Cell Number in Vial (cells/1ml) | 4.2 x 10^6 |
| Viability at cell freezing (%) | 93 | Antibiotics Used | free |
| Passage Number | P28 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmes as human by NP, G6PD, LD |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0823 | Cell Name | YMB-1 |
|---|---|---|---|
| LOT No. | 121096 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% heat inactivated fetal bovine serum (JRH Biosci.1B1062). | Temperature | 37 C |
| Cell Density at Seeding | 1-2 x 10^5 cells/ml | Methods for Passages | Wash cells by PBS(-) and treat with 0.02% EDTA and 0.25% trypsin for 5 min. at 37 C. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.9x10^6 |
| Viability at cell freezing (%) | 98.0 | Antibiotics Used | free |
| Passage Number | P24 | PDL | NT |
| Sterility: MYCOPLASMA | NT | Sterility: BACTERIA | NT |
| Sterility: FUNGI | NT | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO. | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB0823 | Cell Name | |
|---|---|---|---|
| LOT No. | 091195 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% fetal calf serum collagen-coatした培養基を使うとやや接着性があがる | Temperature | 37 |
| Cell Density at Seeding | split ratio=1:6 | Methods for Passages | 0.25% trypsin:接着性は低い。またﾄﾘﾌﾟｼﾝ処理に弱い。処理は短めにし、剥れ始めたらすぐにﾋﾟﾍﾟｯﾃｨﾝｸﾞする事 |
| Doubling Time | Cell Number in Vial (cells/1ml) | ||
| Viability at cell freezing (%) | Antibiotics Used | ||
| Passage Number | PDL | ||
| Sterility: MYCOPLASMA | Sterility: BACTERIA | ||
| Sterility: FUNGI | Isozyme Analysis | ||
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0823 | Cell Name | YMB-1 |
|---|---|---|---|
| LOT No. | 10202020 | Lot Specification | distribution |
| Medium | RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) | Temperature | 37 C |
| Cell Density at Seeding | 0.3 - 1.1 x 10^5 cells/mL | Methods for Passages | Cells are loosely adherent, and attachment is poor. Cells were either disparsed by pipetting or by treatment with 0.25% trypsin and 0.02% EDTA-PBS. |
| Doubling Time | approx. 2.5 days. | Cell Number in Vial (cells/1ml) | 5.3 x 10^6 |
| Viability at cell freezing (%) | 90 | Antibiotics Used | free |
| Passage Number | P31 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI 1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 90 | Additional information |