JCRB0827 VMRC-RCZ

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0827 Cell Name   VMRC-RCZ
Profile   renal carcinoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   kidney
Case history    Metastasis   Yes
Tissue Metastasized    Genetics   renal cancer
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   renal cancer
Classify   tumor Established by   Akiyama,M.
Registered by   Akiyama,M. Regulation for Distribution   
Comment    Year   1988
Medium   Eagle's minimal essential medium with 10% fetal calf serum. Methods for Passages   Cells are treated with 0.25% trypsin and dilute in fresh medium. Change medium twice a week.
Cell Number on Passage   split ratio=1/6 Race   
CO2 Conc.   5 % Tissue Sampling   renal
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1791
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 909781
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LOT Information

Cell No.   JCRB0827 Cell Name   VMRC-RCZ
LOT No.   111988 Lot Specification   distribution
Medium   comp. MEM Temperature   37
Cell Density at Seeding   split 1:6 Methods for Passages   
Doubling Time   NT Cell Number in Vial (cells/1ml)   1-2x10^6
Viability at cell freezing (%)   95% Antibiotics Used   P/S
Passage Number   * PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0827 Cell Name   
LOT No.   091195 Lot Specification   distribution
Medium   Eagle's MEM with 10% fetal calf serum Temperature   37
Cell Density at Seeding   split ratio=1:6 Methods for Passages   0.25% trypsin. Change medium twice a week.
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0827 Cell Name   VMRC-RCZ
LOT No.   111397 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   3-5 x 10^4 cells/ml Methods for Passages   0.25% trypsin
Doubling Time    Cell Number in Vial (cells/1ml)   4.8 x 10^5
Viability at cell freezing (%)   75.0 Antibiotics Used   free
Passage Number   P29 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,12
D13S317:13
D7S820:10,11
D16S539:11
VWA:16
TH01:9.3
AM:X
TPOX:8
CSF1PO:10,12
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0827 Cell Name   VMRC-RCZ
LOT No.   03172020 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 53 hrs. Cell Number in Vial (cells/1ml)   4.3 x 10^5
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   P28 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   95 Additional information   
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