JCRB0828 HO-1-u-1

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0828 Cell Name   HO-1-u-1
Profile   squamous carcinoma, mouth floor Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   72
Identity   available Tissue for Primary Cancer   oral
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span    Crisis PDL   
Morphology    Character   squamous cell carcinoma
Classify    Established by   Miyauchi,S. & Takada,K.
Registered by   Akiyama,M Regulation for Distribution   
Comment    Year   1988
Medium   Dulbecco's modified Eagle's medium - F12 medium (1:1 mix) with 10% fetal calf serum. Methods for Passages   Cells are treated with 0.1 % trypsin and 0.02 % EDTA. Dilute in fresh medium.
Cell Number on Passage   split ratio=1/6 Race   
CO2 Conc.   5 % Tissue Sampling   mouth floor
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2784
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 753561
Reference
Pubmed id:2997498 [An autopsy case of triple cancer associated with superior vena cava syndrome].
Sugiyama A,Ishiguro M,Yasue T,Watanabe S,Sugishita N,Yokoyama H,Uno T,Watanabe H,Matsuno Y,Sasaoka I
Gan No Rinsho. 1985 Aug;31(10):1347-52

Images
Movies



LOT Information

Cell No.   JCRB0828 Cell Name   HO-1-u-1
LOT No.   111988 Lot Specification   distribution
Medium   DM-F12 + 10 % FBS Temperature   37
Cell Density at Seeding   1:6 Methods for Passages   0.1 % trypsin + 0.02 % EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   1-2x10^6
Viability at cell freezing (%)   95% Antibiotics Used   P/S
Passage Number   * PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0828 Cell Name   
LOT No.   080995 Lot Specification   distribution
Medium   DM-F12 + 10 % FBS Temperature   37
Cell Density at Seeding   1:6 Methods for Passages   0.1 % trypsin + 0.02 % EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0828 Cell Name   
LOT No.   090495 Lot Specification   distribution
Medium   DM-F12 + 10 % FBS Temperature   37
Cell Density at Seeding   1:6 Methods for Passages   0.1 % trypsin + 0.02 % EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0828 Cell Name   
LOT No.   10172000 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium : Ham's F12 medium = 1:1 with10% fetal calf serum Temperature   37 C
Cell Density at Seeding    Methods for Passages   0.1% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   P83 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0828 Cell Name   HO-1-u-1
LOT No.   05262014 Lot Specification   distribution
Medium   1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   2.2 - 6.2 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   3.6 x 10^6
Viability at cell freezing (%)   62.1 Antibiotics Used   free
Passage Number   P84 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10
D13S317:9
D7S820:9,10
D16S539:12
VWA:17,19
TH01:9
AM:X,Y
TPOX:10
CSF1PO:10
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   62.1
Additional information   
CLOSE