JCRB9002 BW5147.G.1.4.OUA-R.1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9002 Cell Name   BW5147.G.1.4.OUA-R.1
Profile   lymphoma, T cell lymphoma, (AKR/J mouse) Other Name   BW5147
Animal   mouse Strain   AKR/J
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   
Tissue Metastasized    Genetics   T cell lymphoma
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   
Classify    Established by   Hyman,R.
Registered by   Mizusawa,H. Regulation for Distribution   
Comment   Prepared from CRL 1588. Year   1985
Medium   Dulbecco's modified Eagle's medium with 4.5 g/L glucose and 10% horse serum. Methods for Passages   Dilution. Medium should be replaced on day 3 or 4.
Cell Number on Passage   1-5 x 10^4 cells/60mm dish Race   
CO2 Conc.   5 % Tissue Sampling   
Tissue Type   lymphoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4137
Reference
Pubmed id:6067662L-asparagine biosynthesis by nutritional variants of the Jensen sarcoma.
Patterson MK Jr,Orr G
Biochem Biophys Res Commun. 1967 Jan 23;26(2):228-33
Pubmed id:14403619Primary cultivation and continuous propagation in vitro of tissues from small biopsy specimens.
HSU TC,KELLOGG DS Jr
J Natl Cancer Inst. 1960 Aug;25():221-35


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Pubmed id:26749212 Eomesodermin promotes interferon-γ expression and binds to multiple conserved noncoding sequences across the Ifng locus in mouse thymoma cell lines.
Fukuoka N,Harada M,Nishida A,Ito Y,Shiota H,Kataoka T
Genes Cells. 2016 Feb;21(2):146-62

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LOT Information

Cell No.   JCRB9002 Cell Name   
LOT No.   06212000 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (4.5 g/L glucose) and 10% horse serum Temperature   37 C
Cell Density at Seeding   5-6 x 10^4 cells/ml Methods for Passages   dilution or 0.25% trypsin and 0.02% EDTA (a portion of cells may attach to dish.)
Doubling Time   ca 23 hrs Cell Number in Vial (cells/1ml)   1.3 x 10^7
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium containing 10% DMSO and 20% horse serum CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9002 Cell Name   BW5147.G.1.4.OUA-R.1
LOT No.   073094 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 4.5g/l glucose and 10% horse serum(JRH,2G2141) Temperature   37 C
Cell Density at Seeding   3x10^6 cells/100mm dish Methods for Passages   Cells were suspended by dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.72x10^6
Viability at cell freezing (%)   100 Antibiotics Used   free
Passage Number   P4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   mouse (NP,G6PD,PepB,MPI,AST,LDH)
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9002 Cell Name   BW5147.G.1.4.OUA-R.1
LOT No.   021486 Lot Specification   distribution
Medium   DMEM w/ 4.5 G/L glucose + horse serum 10% Temperature   37
Cell Density at Seeding   1-5 x 10^4/6D Methods for Passages   Dilution. Medium should be replaced on day 3 or 4.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.6x10^4
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P3* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9002 Cell Name   
LOT No.   072994 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 4.5g/l glucose and 10% horse serum(JRH,2G2141) Temperature   37 C
Cell Density at Seeding    Methods for Passages   Cells were suspended by dilution.
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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