JCRB9003 NCTC Clone 929

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9003 Cell Name   NCTC Clone 929
Profile   fibroblast, subclone of strain L, (C3H/An mouse) Other Name   
Animal   mouse Strain   
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   skin
Case history    Metastasis   
Tissue Metastasized    Genetics   connective tissue, clone of strain L.
Life Span    Crisis PDL   
Morphology    Character   connective tissue, clone of strain L.
Classify    Established by   Sanfod et al.
Registered by   Mizusawa,H. Regulation for Distribution   
Comment   Prepared from CCL 1. Year   1985
Medium   MEM + non essential amino acids + horse serum 10% Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   split ratio=1/2 - 1/8 Race   
CO2 Conc.   5 % Tissue Sampling   
Tissue Type   connective tissue

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0462
Reference
Pubmed id:178611 Establishment and characterization of a human histiocytic lymphoma cell line (U-937).
Sundström C,Nilsson K
Int J Cancer. 1976 May 15;17(5):565-77
Pubmed id:13655076 Rapid proliferation of sublines of NCTC clone 929 (strain L) mouse cells in a simple chemically defined medium (MB 752/1).
WAYMOUTH C
J Natl Cancer Inst. 1959 May;22(5):1003-17
Pubmed id:13271674 The minimum vitamin requirements of the L and HeLa cells in tissue culture, the production of specific vitamin deficiencies, and their cure.
EAGLE H
J Exp Med. 1955 Nov 1;102(5):595-600
Pubmed id:13035407 A quantitative study of the effect of certain chemically defined media on the proliferation in vitro of strain L cells from the mouse.
EVANS VJ,SHANNON JE Jr,BRYANT JC,WALTZ HK,EARLE WR,SANFORD KK
J Natl Cancer Inst. 1953 Feb;13(4):773-84

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LOT Information

Cell No.   JCRB9003 Cell Name   
LOT No.   11172008 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% horse serum Temperature   37 C
Cell Density at Seeding   1.5 - 2.0 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time    Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   96.4 Antibiotics Used   free
Passage Number   P565 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% HS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9003 Cell Name   NCTC Clone 929
LOT No.   F00953 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% horse serum(ICN,lot.3110143) Temperature   37 C
Cell Density at Seeding    Methods for Passages   Cells were treated with 0.02% EDTA and 0.25% trypsin (Difco 1:250).
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.7x10^6
Viability at cell freezing (%)   88.1 Antibiotics Used   free
Passage Number   P565 PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9003 Cell Name   NCTC Clone 929
LOT No.   032591 Lot Specification   distribution
Medium   MEM + non essential amino acids + horse serum 10% Temperature   37 C
Cell Density at Seeding   1/2 - 1/8 Methods for Passages   Harvest cells by scraping and adjust concentration of cells.
Doubling Time   NT Cell Number in Vial (cells/1ml)   8.4x10^6
Viability at cell freezing (%)   96% Antibiotics Used   free
Passage Number   P564 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,NP,LD
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9003 Cell Name   NCTC Clone 929
LOT No.   092586 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non essential amino acids and 10% hose serum Temperature   37 C
Cell Density at Seeding   Split ratio = 1/2 - 1/8 Methods for Passages   Harvest cells by scraping and adjust concentration of cells.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.4x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P563 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9003 Cell Name   NCTC Clone 929
LOT No.   060394 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% horse serum(ICN-Flow0130293) and non-essential AAs(GIBCO) Temperature   37 C
Cell Density at Seeding   2-4x10^4 cells/sq.cm Methods for Passages   Cells were harvested by scraping by rubber policeman and diluted by fresh medium including serum.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.87x10^6
Viability at cell freezing (%)   89.3 Antibiotics Used   free
Passage Number   P565 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LDH,NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9003 Cell Name   NCTC Clone 929
LOT No.   09022014 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% horse serum Temperature   37 C
Cell Density at Seeding   1.2 - 1.5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 32 hrs Cell Number in Vial (cells/1ml)   3.4 x 10^6
Viability at cell freezing (%)   84.0 Antibiotics Used   free
Passage Number   P564 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% HS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   84.0
Additional information   
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