JCRB9005 BALB/3T3 clone A31

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9005 Cell Name   BALB/3T3 clone A31
Profile   fibroblast, contact inhibited, (BALB/c mouse) Other Name   BALB/3T3
Animal   mouse Strain   BALB/c
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span    Crisis PDL   
Morphology    Character   contact inhibited
Classify    Established by   Aaronson & Todaro
Registered by   Mizusawa,H. Regulation for Distribution   
Comment   Prepared from CCL163. Year   1985
Medium   Dulbecco's modified Eagle's medium with 10% calf serum Methods for Passages   0.02% EDTA & 0.25% trypsin. Subculture every 3 days. Never allow cultures to become completely confluent.
Cell Number on Passage   3x10^5 cells/60mm dish Race   
CO2 Conc.   5 % Tissue Sampling   embryo
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0184
Reference
Pubmed id:7394516Cell variants showing differential susceptibility to ultraviolet light--induced transformation.
Kakunaga T,Crow JD
Science. 1980 Jul 25;209(4455):505-7
Pubmed id:4311790Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.
Jainchill JL,Aaronson SA,Todaro GJ
J Virol. 1969 Nov;4(5):549-53
Pubmed id:4301006Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40.
Aaronson SA,Todaro GJ
J Cell Physiol. 1968 Oct;72(2):141-8
Pubmed id:13985244Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines.
TODARO GJ,GREEN H
J Cell Biol. 1963 May;17():299-313

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LOT Information

Cell No.   JCRB9005 Cell Name   
LOT No.   11202009 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 2 - 3 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   2.1 x 10^6
Viability at cell freezing (%)   93.7 Antibiotics Used   free
Passage Number   P72 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9005 Cell Name   
LOT No.   9005 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum Temperature   37C
Cell Density at Seeding   1 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in PBS
Doubling Time   16hr Cell Number in Vial (cells/1ml)   1.2x 10^5
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   P72 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   70% Culture medium + 20% FCS + 10% DMSO CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9005 Cell Name   
LOT No.   12102007 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum Temperature   37C
Cell Density at Seeding   1x10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in PBS
Doubling Time   16hr Cell Number in Vial (cells/1ml)   6.0 x 10^5
Viability at cell freezing (%)   91 Antibiotics Used   free
Passage Number   P72 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   70% Culture medium + 20% CS + 10% DMSO CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9005 Cell Name   
LOT No.   08072003 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum Temperature   37 C
Cell Density at Seeding   2 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T. = ca. 24 hrs Cell Number in Vial (cells/1ml)   7.3 x 10^5
Viability at cell freezing (%)   92.7 Antibiotics Used   free
Passage Number   P72 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9005 Cell Name   BALB/3T3 clone A31
LOT No.   111695 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's minimal medium with 10% calf serum (ICN Flow 1059023). Temperature   37 C
Cell Density at Seeding   8.4x10^4 cells/ml Methods for Passages   Wash cells with PBS(-) and treated with 0.25%-trypsin and 0.02% EDTA for 10min. at 37 C.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.9x10^6
Viability at cell freezing (%)   97.0 Antibiotics Used   free
Passage Number   P70 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9005 Cell Name   BALB/3T3 clone A31
LOT No.   F00963 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum (BioWhittaker, 3MC0581) Temperature   37 C
Cell Density at Seeding   2.5-3.2x10^4 cells/ml Methods for Passages   Cells were treated with 0.02% EDTA and 0.25% trypsin (Difco 1:250)
Doubling Time   NT Cell Number in Vial (cells/1ml)   7.2x10^5
Viability at cell freezing (%)   77.2 Antibiotics Used   free
Passage Number   P69 PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9005 Cell Name   BALB/3T3 clone A31
LOT No.   090993 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum (Hyclone, 2111480) Temperature   37 C
Cell Density at Seeding   7-8x10^4 cells/ml Methods for Passages   Wash cells with PBS(-) and treated with 0.25% trypsin and 0.02% EDTA for 5-15min. at 37 C.
Doubling Time   15.5 hours. Cell Number in Vial (cells/1ml)   1.5x10^6
Viability at cell freezing (%)   96.5 Antibiotics Used   free
Passage Number   P70 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   NT Isozyme Analysis   G6PD, LD, Pep B
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9005 Cell Name   BALB/3T3 clone A31
LOT No.   082493 Lot Specification   distribution
Medium   Cells discarded. Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   G6PD, LD, PepB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9005 Cell Name   BALB/3T3 clone A31
LOT No.   101691 Lot Specification   distribution
Medium   DMEM + CS 10% Temperature   37 C
Cell Density at Seeding   3x10^5/6D Methods for Passages   0.02% EDTA and 0.25% trypsin. Subculture every 3 days. Never allow cultures to become completely confluent.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.0x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P71 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9005 Cell Name   BALB/3T3 clone A31
LOT No.   100890 Lot Specification   distribution
Medium   DMEM + CS 10% Temperature   37
Cell Density at Seeding   3x10^5/6D Methods for Passages   0.02% EDTA and 0.25% trypsin. Subculture every 3 days. Never allow cultures to become completely confluent.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.5x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P71 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9005 Cell Name   BALB/3T3 clone A31
LOT No.   090888 Lot Specification   distribution
Medium   DMEM + CS 10% Temperature   37
Cell Density at Seeding   3x10^5/6D Methods for Passages   0.02% EDTA and 0.25% trypsin. Subculture every 3 days. Never allow cultures to become completely confluent.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P70 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9005 Cell Name   BALB/3T3 clone A31
LOT No.   03212013 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099). The vial is incorrectly labeled as 03262013. Temperature   37 C
Cell Density at Seeding   1 - 2.4 x 10^4 cells / mL Methods for Passages   0.02% EDTA & 0.25% trypsin. Subculture every 3 days. Never allow cultures to become completely confluent.
Doubling Time    Cell Number in Vial (cells/1ml)   7.1 x 10^5
Viability at cell freezing (%)   98.6 Antibiotics Used   free
Passage Number   p72 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   10% DMSO, 20% FBS, DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   98.6 Additional information   
Cell No.   JCRB9005 Cell Name   BALB/3T3 clone A31
LOT No.   10062016 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 3.5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Never allow cultures to become completely confluent.
Doubling Time   approx. 28 hrs Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   P72 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   97 Additional information   
Cell No.   JCRB9005 Cell Name   BALB/3T3 clone A31
LOT No.   08062021 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; BioWest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   4.2 - 8.2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 31 hrs. Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   99.2 Antibiotics Used   free
Passage Number   P72 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   5% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   96.0 Additional information   
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