JCRB9007 VERO 76

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9007 Cell Name   VERO 76
Profile   normal, contact inhibited, lower saturation density than the original Vero Other Name   
Animal   monkey, African green Strain   African green
Genus   Cercopithecus Species   aethiops
Sex    Age   
Identity    Tissue for Primary Cancer   kidney
Case history    Metastasis   
Tissue Metastasized    Genetics   kidney
Life Span    Crisis PDL   
Morphology    Character   kidney
Classify    Established by   
Registered by   Mizusawa,H. Regulation for Distribution   
Comment   Prepared from CRL 1587. Year   1985
Medium   Dulbecco's modified Eagle's medium or Eagle's minimal essential medium with 10% fetal calf serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   split ratio=1/8 Race   
CO2 Conc.   5 % Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0603
Reference
Pubmed id:2725683Mycoplasma infection of cultured cells.
Hay RJ,Macy ML,Chen TR
Nature. 1989 Jun 8;339(6224):487-8
Pubmed id:4290661SV40 transformation of human diploid cells. A parallel study of viral and karyologic parameters.
Girardi AJ,Weinstein D,Moorhead PS
Ann Med Exp Biol Fenn. 1966;44(2):242-54
Pubmed id:5833688Studies on measles virus. II. Propagation in two established simian renal cell lines and development of a plaque assay.
Sasaki K,Makino S,Kasahara S
Kitasato Arch Exp Med. 1964 Dec;37(1):27-42




LOT Information

Cell No.   JCRB9007 Cell Name   VERO 76
LOT No.   041387 Lot Specification   distribution
Medium   DMEM or MEM + FCS 10% Temperature   37
Cell Density at Seeding   1/8 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.7x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P32 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9007 Cell Name   VERO 76
LOT No.   09072015 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   approx. 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   25 - 44 hrs Cell Number in Vial (cells/1ml)   5.6 x 10^6
Viability at cell freezing (%)   90.2 Antibiotics Used   free
Passage Number   P33 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   90.2 Additional information   
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