JCRB9008 MRC-5

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9008 Cell Name   MRC-5
Profile   fetal lung, normal diploid fibroblast Other Name   MRC-5
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   14-weeks
Identity   available Tissue for Primary Cancer   lung
Case history    Metastasis   
Tissue Metastasized    Genetics   embryonal lung, diploid, male.
Life Span    Crisis PDL   
Morphology    Character   embryonal lung, diploid, male.
Classify    Established by   Jacobs,J.P.
Registered by   Mizusawa,H. Regulation for Distribution   
Comment   Prepared from CCL 171. Population doubling is 23 at passage No.18. Year   1985
Medium   Basal medium Eagle with 10% fetal calf serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   split ratio=1/2 - 1/5 Race   
CO2 Conc.   5 % Tissue Sampling   fetus lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0440
Reference
Pubmed id:7389832 A new human diploid cell strain, TIG-1, for the research on cellular aging.
Ohashi M,Aizawa S,Ooka H,Ohsawa T,Kaji K,Kondo H,Kobayashi T,Noumura T,Matsuo M,Mitsui Y,Murota S,Yamamoto K,Ito H,Shimada H,Utakoji T
Exp Gerontol. 1980;15(2):121-33
Pubmed id:12635262 Altered enzymes in ageing human fibroblasts.
Holliday R,Tarrant GM
Nature. 1972 Jul 7;238(5358):26-30
Pubmed id:12635218 Error theory and ageing in human diploid fibroblasts.
Lewis CM,Tarrant GM
Nature. 1972 Oct 6;239(5371):316-8
Pubmed id:4316953 Characteristics of a human diploid cell designated MRC-5.
Jacobs JP,Jones CM,Baille JP
Nature. 1970 Jul 11;227(5254):168-70

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LOT Information

Cell No.   JCRB9008 Cell Name   MRC-5
LOT No.   06022011 Lot Specification   distribution
Medium   Basal medium Eagle with 10% fetal bovine serum (GIBCO Cat. # 10099). You can use Eagle's MEM instead of BME. Temperature   37 C
Cell Density at Seeding   approx. 5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 34 hrs. Cell Number in Vial (cells/1ml)   1.0 x 10^6
Viability at cell freezing (%)   89.5 Antibiotics Used   free
Passage Number   P23 PDL   35
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,12
D13S317:11,14
D7S820:10,11
D16S539:9,11
VWA:15
TH01:8
AM:X,Y
TPOX:8
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - BME. CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   Expected viability (defrost ampoule) 96 %.
Cell No.   JCRB9008 Cell Name   
LOT No.   03172009 Lot Specification   distribution
Medium   Basal Medium Eagle with 10% fetal bovine serum (GIBCO Cat. # 10099, Lot 484375) Temperature   37 C
Cell Density at Seeding   approx. 3 - 5 x 10^4 cells/ml (1:6 split) Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   86.3 Antibiotics Used   free
Passage Number   P23 PDL   38
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - BME CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9008 Cell Name   
LOT No.   05202005 Lot Specification   distribution
Medium   Basal medium Eagle with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   approx. 5 x 10^4 cells/ml (1 x 10^4/cm2) Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 1.5 days Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   93.1 Antibiotics Used   free
Passage Number   P23 PDL   35
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - BME CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9008 Cell Name   
LOT No.   05192003 Lot Specification   distribution
Medium   Basal medium Eagle (Earle's salts) with 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   4-5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA.
Doubling Time   D.T. = ca. 2 days Cell Number in Vial (cells/1ml)   9.2 x 10^5
Viability at cell freezing (%)   95.2 Antibiotics Used   free
Passage Number   P23 PDL   35
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-BME CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9008 Cell Name   MRC-5
LOT No.   040296 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number    PDL   35
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as a human by LDH, NP, G6PD (human B).
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB9008 Cell Name   MRC-5
LOT No.   111793 Lot Specification   distribution
Medium   Basal medium Eagle with 10% FBS(Hyclone,1151031) (BME=Gibco Powdered, autoclavable 65k5431) Temperature   37 C
Cell Density at Seeding   9x10^5 cells/10cm dish Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.5x10^6
Viability at cell freezing (%)   90.2 Antibiotics Used   free
Passage Number   P23 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9008 Cell Name   MRC-5
LOT No.   041392 Lot Specification   distribution
Medium   B.MEM(lot.030292) + 10% FBS(lot.J.R.Scientific D10400) Temperature   37 C
Cell Density at Seeding   1.2x10^6 Methods for Passages   0.25% trypsin in 0.02% EDTA
Doubling Time   36hrs. Cell Number in Vial (cells/1ml)   3.1x10^6
Viability at cell freezing (%)   95.1 Antibiotics Used   free
Passage Number   P24 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9008 Cell Name   MRC-5
LOT No.   021089 Lot Specification   distribution
Medium   BME + FCS 10% Temperature   37
Cell Density at Seeding   1/2 - 1/5 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.5x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P23 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9008 Cell Name   
LOT No.   031098 Lot Specification   distribution
Medium   Basal medium Eagle with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   1-2 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA, split 1:2-1:5. Despite of 33 PDL, cells grow very slowly.
Doubling Time   ca. 5 days Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   93.0 Antibiotics Used   free
Passage Number   P24 PDL   33
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9008 Cell Name   MRC-5
LOT No.   04212016 Lot Specification   distribution
Medium   Basal medium Eagle with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   7-8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Split ratio = 1/4.
Doubling Time   approx. 38 hrs. Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   85.0 Antibiotics Used   free
Passage Number   P23 PDL   PDL=35
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS -BME CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   85.0
Additional information   
Cell No.   JCRB9008 Cell Name   MRC-5
LOT No.   02012018 Lot Specification   distribution
Medium   Basal medium Eagle with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   0.9 - 1.7 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 29 hrs. Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   86.2 Antibiotics Used   free
Passage Number   P23 PDL   PDL=35.1
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - basal medium Eagle CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   86.2
Additional information   
Cell No.   JCRB9008 Cell Name   MRC-5
LOT No.   12132019 Lot Specification   distribution
Medium   Basal medium Eagle (SIGMA,Ca.No.;B1522) with 10% heat inactivated fetal bovine serum (SIGMA Cat. No. 172012,Lot;12J396). Temperature   37 C
Cell Density at Seeding   1.0-3.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(Split ratio=1/2-1/5)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.13x10^6
Viability at cell freezing (%)   99.30 Antibiotics Used   free
Passage Number   p23 PDL   35.828
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:11,14
D7S820:10,11
D16S539:9,11
VWA:15
TH01:8
AM:X,Y
TPOX:8
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information   
Images
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