JCRB9009 A-431

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9009 Cell Name   A-431
Profile   epidermoid carcinoma, high expression of EGF-receptor Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   85
Identity    Tissue for Primary Cancer   epidermoid carcinoma
Case history    Metastasis   
Tissue Metastasized    Genetics   epidermoid carcinoma
Life Span    Crisis PDL   
Morphology    Character   epidermoid carcinoma
Classify    Established by   Giard & Aaronson
Registered by   Mizusawa,H. Regulation for Distribution   
Comment   Prepared from CRL1555. Year   1985
Medium   Dulbecco's modified Eagle's medium with 4.5g/L glucose and fetal calf serum Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   split ratio=1/3 - 1/8 Race   
CO2 Conc.   5 % Tissue Sampling   epiderm
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0037
Reference
Pubmed id:2459591The epidermal growth factor- and interferon-independent effects of double-stranded RNA in A431 cells.
Chapekar MS,Knode MC,Glazer RI
Mol Pharmacol. 1988 Oct;34(4):461-6
Pubmed id:2458340Differential effects of in vitro mycoplasma infection on interleukin-1 alpha and beta mRNA expression in U937 and A431 cells.
Demczuk S,Baumberger C,Mach B,Dayer JM
J Biol Chem. 1988 Sep 15;263(26):13039-45
Pubmed id:3526330yes-related protooncogene, syn, belongs to the protein-tyrosine kinase family.
Semba K,Nishizawa M,Miyajima N,Yoshida MC,Sukegawa J,Yamanashi Y,Sasaki M,Yamamoto T,Toyoshima K
Proc Natl Acad Sci U S A. 1986 Aug;83(15):5459-63
Pubmed id:4357758In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.
Giard DJ,Aaronson SA,Todaro GJ,Arnstein P,Kersey JH,Dosik H,Parks WP
J Natl Cancer Inst. 1973 Nov;51(5):1417-23

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LOT Information

Cell No.   JCRB9009 Cell Name   A-431
LOT No.   080594 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 4.5g/l glucose and 10% fetal bovine serum /Bio cell, 4001776 Temperature   37 C
Cell Density at Seeding   2-3x10^5 cells/ml Methods for Passages   Cells were harvested after the treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   9.3x10^5
Viability at cell freezing (%)   98.4 Antibiotics Used   free
Passage Number   P28 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   human (NP,G6PD,PepB,MPI,AST)
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9009 Cell Name   A-431
LOT No.   F00954 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (M.A.Bioproducts, lot.99965) Temperature   37 C
Cell Density at Seeding    Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin (Difco 1:250).
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.6x10^6
Viability at cell freezing (%)   91.9 Antibiotics Used   free
Passage Number   P29 PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
D13S317:9,13
D7S820:10
D16S539:12,14
VWA:15,17
TH01:9
AM:X
TPOX:11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9009 Cell Name   A-431
LOT No.   022592 Lot Specification   distribution
Medium   D.MEM (lot.120291 with glucose 4.5g/l)+FBS 10 % (J.R. Scientific CO19407) Temperature   37 C
Cell Density at Seeding   1x10^6 Methods for Passages   0.02 % EDTA + 0.25 % trypsin
Doubling Time   24 hr Cell Number in Vial (cells/1ml)   1.3x10^6
Viability at cell freezing (%)   90.7 Antibiotics Used   free
Passage Number   P30 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9009 Cell Name   A-431
LOT No.   122589 Lot Specification   distribution
Medium   DMEM ( glucose 4.5g/l ) + FCS 10% Temperature   37
Cell Density at Seeding   1/3 - 1/8 Methods for Passages   0.02% EDTA and 0.25% Trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.3x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P29 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9009 Cell Name   A-431
LOT No.   021086 Lot Specification   distribution
Medium   DMEM ( glucose 4.5g/l ) + FCS 10% Temperature   37
Cell Density at Seeding   1/3 - 1/8 Methods for Passages   0.02% EDTA and 0.25% Trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P28 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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