JCRB9010 HeLa S3

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9010 Cell Name   HeLa S3
Profile   cervical epitheloid carcinoma, adaptable to a suspension culture Other Name   HeLa
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   31
Identity   available Tissue for Primary Cancer   uterine cervix
Case history   cervix cancer Metastasis   
Tissue Metastasized    Genetics   epitheloid carcinoma, cervix.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   adaptable to a suspension culture
Classify   tumor Established by   Puck,Marcus,Cieciura
Registered by   Mizusawa,H. Regulation for Distribution   
Comment   Prepared from CCL 2.2. Year   1985
Medium   Ham's F-12 medium with 10% fetal bovine serum Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   split ratio=1/2 - 1/10 Race   Black
CO2 Conc.   5 % Tissue Sampling   cervix
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 +
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0058
Reference
Pubmed id:11438719 Cell culture forensics.
O'Brien SJ
Proc Natl Acad Sci U S A. 2001 Jul 3;98(14):7656-8
Pubmed id:9892199 Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping.
Macville M,Schröck E,Padilla-Nash H,Keck C,Ghadimi BM,Zimonjic D,Popescu N,Ried T
Cancer Res. 1999 Jan 1;59(1):141-50
Pubmed id:9215212 Record of the first physician to see Henrietta Lacks at the Johns Hopkins Hospital: history of the beginning of the HeLa cell line.
Jones HW Jr
Am J Obstet Gynecol. 1997 Jun;176(6):S227-8
Pubmed id:3691964 History of the acceptance of human diploid cell strains as substrates for human virus vaccine manufacture.
Hayflick L,Plotkin S,Stevenson RE
Dev Biol Stand. 1987;68():9-17
Pubmed id:4361348 Colony formation of mammalian cells on agar plates and its application to Lederberg's replica plating.
Kuroki T
Exp Cell Res. 1973 Jul;80(1):55-62
Pubmed id:5543947 Localization of the replication point of mammalian cell DNA at the membrane.
Hanaoka F,Yamada M
Biochem Biophys Res Commun. 1971 Feb 19;42(4):647-53
Pubmed id:13814142 The effect of cell population density on the amino acid requirements for poliovirus synthesis in HeLa cells.
DARNELL JE Jr,EAGLE H,SAWYER TK
J Exp Med. 1959 Sep 1;110():445-50
Pubmed id:17811344 Molecular Growth Requirements of Single Mammalian Cells.
Sato G,Fisher HW,Puck TT
Science. 1957 Nov 8;126(3280):961-4
Pubmed id:13286432 Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer.
PUCK TT,MARCUS PI,CIECIURA SJ
J Exp Med. 1956 Feb 1;103(2):273-83
Pubmed id:16589695 A RAPID METHOD FOR VIABLE CELL TITRATION AND CLONE PRODUCTION WITH HELA CELLS IN TISSUE CULTURE: THE USE OF X-IRRADIATED CELLS TO SUPPLY CONDITIONING FACTORS.
Puck TT,Marcus PI
Proc Natl Acad Sci U S A. 1955 Jul 15;41(7):432-7
Pubmed id:13669339

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LOT Information

Cell No.   JCRB9010 Cell Name   HeLa S3
LOT No.   08022005 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S). Ampoule label JCRB9110 was mistyped. Correctly JCRB9010. Temperature   37 C
Cell Density at Seeding   5 - 10 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   ca. 70 hr Cell Number in Vial (cells/1ml)   3 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   Mislabeled as JCRB9110. Correctly, JCRB9010.
Cell No.   JCRB9010 Cell Name   HeLa S3
LOT No.   120292 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal calf serum (Flow Lab.0070893) Temperature   37 C
Cell Density at Seeding   1x10^5 cells/ml Methods for Passages   Cells are washed once with PBS, treated with 0.02% EDTA and 0.25% trypsin at 37 C for 15 min.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.61x10^6
Viability at cell freezing (%)   93.5 Antibiotics Used   free
Passage Number   *P7 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:14
D7S820:8,12
D16S539:9,10
VWA:16,18
TH01:7
AM:X
TPOX:8,12
CSF1PO:9,10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB9010 Cell Name   HeLa S3
LOT No.   031491 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal calf serum. Temperature   37 C
Cell Density at Seeding   1/2 - 1/10 Methods for Passages   0.02% EDTA and 0.25% Trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.4x10^6
Viability at cell freezing (%)   95% Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9010 Cell Name   HeLa S3
LOT No.   101086 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal calf serum. Temperature   37 C
Cell Density at Seeding   1/2 - 1/10 Methods for Passages   0.02% EDTA and 0.25% Trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   7x10^5/ml
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P9* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9010 Cell Name   
LOT No.   070997 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal bovine serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   1-2 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   6.5 x 10^5
Viability at cell freezing (%)   86.0 Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9010 Cell Name   HeLa S3
LOT No.   08202013 Lot Specification   distribution
Medium   Ham's F-12 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099). Temperature   37 C
Cell Density at Seeding   1.0 - 1.3 x 10^5 cells mL Methods for Passages   Adheren and non-adherant cells exist. Collect both types of cells (the adherent cells were harvested by 0.02% EDTA and 0.25% trypsin treatment), and resuspend to fresh medium.
Doubling Time    Cell Number in Vial (cells/1ml)   1.4 x 10^6 cells/1mL
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   81.9
Additional information   
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