JCRB9014 3T3 L1

Cell information

Important Notice(s)

3T3-L1、登録番号IFO50416とJCRB9014との違い/ Difference in JCRB9014 3T3-L1 and IFO50416 3T3 L1

3T3 L1細胞の解凍、培養に関する注意 / Note for culturing 3T3-L1.

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9014 Cell Name   3T3 L1
Profile   Clonal subline of the mouse 3T3 that accumulate large amounts of triglyceride fat when the cells are in the resting state. Other Name   
Animal   mouse Strain   Swiss albino
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   embryo
Case history   normal Metastasis   
Tissue Metastasized    Genetics   differentiate to adipocyte
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   The 3T3 L1 is a clonal subline of mouse 3T3 cells.
Classify   normal Established by   Green,H. et al.
Registered by   Mizusawa,H. Regulation for Distribution   
Comment    Year   1985
Medium   Dulbecco's modified Eagle's medium with 10 % calf serum Methods for Passages   0.02% EDTA and 0.25% Trypsin. Never allow culture to become completely confluent & subculture ever 3days.
Cell Number on Passage   2-10 x 10^3 cells/cm2 Race   
CO2 Conc.   5 % Tissue Sampling   embryo
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0123
Reference
Pubmed id:12647936Characterization of heterokaryons between skeletal myoblasts and preadipocytes: myogenic potential of 3T3-L1 preadipocytes.
Sasao N,Hirayama E,Kim J
Eur J Cell Biol. 2003 Feb;82(2):97-103
Pubmed id:313567Insulin-stimulated protein phosphorylation in 3T3-L1 preadipocytes.
Smith CJ,Wejksnora PJ,Warner JR,Rubin CS,Rosen OM
Proc Natl Acad Sci U S A. 1979 Jun;76(6):2725-9
Pubmed id:81205Development of hormone receptors and hormonal responsiveness in vitro. Insulin receptors and insulin sensitivity in the preadipocyte and adipocyte forms of 3T3-L1 cells.
Rubin CS,Hirsch A,Fung C,Rosen OM
J Biol Chem. 1978 Oct 25;253(20):7570-8
Pubmed id:165899An established preadipose cell line and its differentiation in culture. II. Factors affecting the adipose conversion.
Green H,Kehinde O
Cell. 1975 May;5(1):19-27


Research results by users. Click！
Pubmed id:28811612 Coenzyme Q10 Improves Lipid Metabolism and Ameliorates Obesity by Regulating CaMKII-Mediated PDE4 Inhibition.
Xu Z,Huo J,Ding X,Yang M,Li L,Dai J,Hosoe K,Kubo H,Mori M,Higuchi K,Sawashita J
Sci Rep. 2017 Aug 15;7(1):8253

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LOT Information

Cell No.   JCRB9014 Cell Name   
LOT No.   11272012 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (1.0 g/L glucose) with 10% newborn calf serum (GIBCO Cat. # 16010) Temperature   37 C
Cell Density at Seeding   approx. 2 x 10^4 cells/mL (4 x 10^3 cells/cm2) Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 43 hrs Cell Number in Vial (cells/1ml)   8.5 x 10^5
Viability at cell freezing (%)   98.8 Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% newborn calf serum - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9014 Cell Name   3T3 L1
LOT No.   02132012 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (1 g/L glucose) with 10% newborn calf serum Temperature   37 C
Cell Density at Seeding   1.2 - 3.0 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 41 hrs Cell Number in Vial (cells/1ml)   4.2 x 10^5
Viability at cell freezing (%)   94.5 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9014 Cell Name   
LOT No.   08222011 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% newborn calf serum (GIBCO Cat. # 16010) Temperature   37 C
Cell Density at Seeding   2 - 3 x 10^4 cells/ml (4-6 x 10^3 cells/cm2) Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 28 hrs Cell Number in Vial (cells/1ml)   8.2 x 10^5
Viability at cell freezing (%)   96.5 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9014 Cell Name   
LOT No.   02152011 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% newborn calf serum (GIBCO Cat. # 16010) Temperature   37 C
Cell Density at Seeding   2 - 3 x 10^4 cells/ml (4-6 x 10^3 cells/cm2) Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 23 hrs Cell Number in Vial (cells/1ml)   2.6 x 10^5
Viability at cell freezing (%)   96.2 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9014 Cell Name   3T3 L1
LOT No.   10182010 Lot Specification   distribution1
Medium   DMEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   5.94-16.9x10^3cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.Never allow culture to become completely confluent and subculture ever 3days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.08x10^6
Viability at cell freezing (%)   94.5 Antibiotics Used   free
Passage Number   p9* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9014 Cell Name   3T3 L1
LOT No.   10272010 Lot Specification   distribution2
Medium   DMEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   5.53-16.9x10^3cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.Never allow culture to become completely confluent and subculture ever 3days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.52x10^6
Viability at cell freezing (%)   95.9 Antibiotics Used   free
Passage Number   p12* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9014 Cell Name   3T3 L1
LOT No.   11042010 Lot Specification   distribution3
Medium   DMEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   5.53-16.9x10^3cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.Never allow culture to become completely confluent and subculture ever 3days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.04x10^6
Viability at cell freezing (%)   97.2 Antibiotics Used   free
Passage Number   p14* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9014 Cell Name   
LOT No.   01142010 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% newborn calf serum (GIBCO Cat. # 16010) Temperature   37 C
Cell Density at Seeding   2 - 3 x 10^4 cells/ml (4-6 x 10^3 cells/cm2) Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 26 hrs Cell Number in Vial (cells/1ml)   5.2 x 10^5
Viability at cell freezing (%)   95.5 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9014 Cell Name   
LOT No.   01282009 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum (GIBCO Cat. # 16010, Lot 655639). The lot number 01282008 labeled on vial is misprint. Correct lot number of this vial is 01282009. Temperature   37 C
Cell Density at Seeding   1.2 - 1.8 x 10^4 cells/ml. Methods for Passages   0.25% trypsin and 0.02% EDTA. Do not arrow to be full confluency.
Doubling Time   approx. 31 hrs Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   95.4 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9014 Cell Name   
LOT No.   01252008 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum (newborn calf serum was used) Temperature   37 C
Cell Density at Seeding   3 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Do not allow the culture full confluency.
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   1.0 x 10^6
Viability at cell freezing (%)   97.8 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9014 Cell Name   
LOT No.   02092007 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum Temperature   37 C
Cell Density at Seeding   2-4 x 10^4 cells/ml (4-8 x 10^3 cells/cm2) Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 1.5 days Cell Number in Vial (cells/1ml)   4.1 x 10^5
Viability at cell freezing (%)   95.1 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD , MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9014 Cell Name   
LOT No.   06122006 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum Temperature   37 C
Cell Density at Seeding   3-5 x 10^4 cells/ml (6-10 x 10^3 cells/sq.cm) Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 33 hrs Cell Number in Vial (cells/1ml)   3.7 x 10^5
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9014 Cell Name   
LOT No.   12162004 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum Temperature   37 C
Cell Density at Seeding   1 - 3 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   ca. 40 hrs Cell Number in Vial (cells/1ml)   3.1 x 10^5
Viability at cell freezing (%)   88 Antibiotics Used   free
Passage Number   P7* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9014 Cell Name   
LOT No.   06082004 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum Temperature   37 C
Cell Density at Seeding   1-2 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time   60 - 70 hrs Cell Number in Vial (cells/1ml)   3.1 x 10^5
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   P7* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9014 Cell Name   
LOT No.   04142000 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum Temperature   37 C
Cell Density at Seeding   7-9 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   5.4 x 10^5
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   P8* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS-DMEM CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9014 Cell Name   3T3 L1
LOT No.   052896 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number   p7* PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9014 Cell Name   3T3 L1
LOT No.   100893 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum (Hyclone,2111480) Temperature   37 C
Cell Density at Seeding   8.0x10^4 cells/ml Methods for Passages   Wash once with PBS(-) and treat with 0.25% trypsin and 0.02% EDTA at 37 C for 8-23 min.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.07x10^6
Viability at cell freezing (%)   98.6 Antibiotics Used   free
Passage Number   p9* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, LD, PepB
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9014 Cell Name   3T3 L1
LOT No.   110891 Lot Specification   distribution
Medium   DMEM + CS 10% Temperature   37 C
Cell Density at Seeding   3x10^5cells/6D. Methods for Passages   0.02% EDTA and 0.25% Trypsin. Never allow culture to become completely confluent and subculture ever
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   p7* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9014 Cell Name   3T3 L1
LOT No.   091886 Lot Specification   distribution
Medium   DMEM + CS 10% Temperature   37 C
Cell Density at Seeding   3x10^5/6D Methods for Passages   0.02% EDTA and 0.25% Trypsin. Never allow culture to become completely confluent and subculture every 3 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.4x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P7* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9014 Cell Name   
LOT No.   10302002 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum Temperature   37 C
Cell Density at Seeding   5 x 10^3 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time   D.T = ca 3 days Cell Number in Vial (cells/1ml)   1.9 x 10^5
Viability at cell freezing (%)   90.5 Antibiotics Used   free
Passage Number   Unknown (7 at cellbank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS in DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9014 Cell Name   
LOT No.   032499 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum Temperature   37 C
Cell Density at Seeding   3-5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T.=ca. 37 hrs Cell Number in Vial (cells/1ml)   1.5 x 10^5
Viability at cell freezing (%)   94.0 Antibiotics Used   free
Passage Number   P8* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9014 Cell Name   
LOT No.   051597 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum (The use of FCS is also possible) Temperature   37 C
Cell Density at Seeding   1-4 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA Do not allow culture confluent at maintenance.
Doubling Time    Cell Number in Vial (cells/1ml)   4.2 x 10^5
Viability at cell freezing (%)   87.0 Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as mouse by NP, G6PD, LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9014 Cell Name   3T3 L1
LOT No.   01202013 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (1.0 g/L glucose) with 10% newborn calf serum (GIBCO Cat. # 16010) Temperature   37 C
Cell Density at Seeding   2.7 - 3.2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   4.5 x 10^5
Viability at cell freezing (%)   90.0 Antibiotics Used   free
Passage Number   Unknown (14 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% newborn calf serum - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   90.0 Additional information   
Cell No.   JCRB9014 Cell Name   3T3 L1
LOT No.   05252015 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (1 g/L glucose type) with 10 % newborn calf serum Temperature   37 C
Cell Density at Seeding   1.8 - 3.2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Avoid to allow the cells to full confluency.
Doubling Time   approx. 33 hrs Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   89.3 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% NCS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   89.3 Additional information   
Cell No.   JCRB9014 Cell Name   3T3 L1
LOT No.   06012015 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10 % newborn calf serum (GIBCO Cat. # 16010). DMEM is 1g/L glucose type and must contain L-glutamine. Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Never allow culture to become full confluency.
Doubling Time   approx. 30 hrs Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   89.0 Antibiotics Used   free
Passage Number   Unknown (13 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% newborn calf serum - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   89.0 Additional information   
Cell No.   JCRB9014 Cell Name   3T3 L1
LOT No.   08222016 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (low glucose) with 10% newborn calf serum (GIBCO Cat. # 16010) Temperature   37 C
Cell Density at Seeding   1.8 - 3.0 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Avoid to allow the cells to full confluency.
Doubling Time   approx. 31 hrs Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   98.2 Antibiotics Used   free
Passage Number   P11* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% newborn calf serum - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   98.2 Additional information   
Cell No.   JCRB9014 Cell Name   3T3 L1
LOT No.   11212017 Lot Specification   distribution-1
Medium   Dulbecco's modified Eagle's medium(GIBCO) with 10 % heat inactivated newborn calf serum(Lot.:C14368S0750) Temperature   37 C
Cell Density at Seeding   0.62-1.8x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.09x10^6
Viability at cell freezing (%)   98.1 Antibiotics Used   free
Passage Number   p10* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   
Images
Cell No.   JCRB9014 Cell Name   3T3 L1
LOT No.   11242017 Lot Specification   distribution-2
Medium   Dulbecco's modified Eagle's medium(GIBCO) with 10 % heat inactivated newborn calf serum(Lot.:C14368S0750) Temperature   37 C
Cell Density at Seeding   0.62-1.8x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.00x10^6
Viability at cell freezing (%)   98.5 Antibiotics Used   free
Passage Number   p11* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Bambanker CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   
Images
Cell No.   JCRB9014 Cell Name   3T3 L1
LOT No.   11302017 Lot Specification   distribution-3
Medium   Dulbecco's modified Eagle's medium(GIBCO) with 10 % heat inactivated newborn calf serum(Lot.:C14368S0750) Temperature   37 C
Cell Density at Seeding   0.55-1.8x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.10x10^6
Viability at cell freezing (%)   99.7 Antibiotics Used   free
Passage Number   p13* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   
Images
Cell No.   JCRB9014 Cell Name   3T3 L1
LOT No.   04212017 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (low glucose) with 10 % newborn calf serum. Temperature   37 C
Cell Density at Seeding   1.4 - 5.1 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Never allow culture to become completely confluent.
Doubling Time   20 - 64 hrs. Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   93.7 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   5% DMSO, 20% NCS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   93.7 Additional information   
Cell No.   JCRB9014 Cell Name   3T3 L1
LOT No.   06182020 Lot Specification   distribution
Medium   DMEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA,Cat#172012,Lot#12J396) Temperature   37 C
Cell Density at Seeding   4.63-5.8x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.11x10^6
Viability at cell freezing (%)   100 Antibiotics Used   free
Passage Number   p10* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   92 Additional information   
Images
Cell No.   JCRB9014 Cell Name   3T3 L1
LOT No.   10082020 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (low glucose) with 10 % newborn calf serum. Temperature   37 C
Cell Density at Seeding   approx. 4.5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Never allow culture to become completely confluent.
Doubling Time   approx. 27 hrs. Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   96.8 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   5% DMSO, 20% newborn calf serum - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   94.3 Additional information   
Cell No.   JCRB9014 Cell Name   3T3 L1
LOT No.   03132023 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (low glucose) with 10% newborn calf serum (NCS; Biowest Cat. # S0750, Lot S14368S0750) Temperature   37 C
Cell Density at Seeding   1.1 - 5.0 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Never allow culture to become full confluency.
Doubling Time   28 - 39 hrs Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   89.8 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   5% DMSO, 20% NCS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   89.8 Additional information   
Cell No.   JCRB9014 Cell Name   3T3 L1
LOT No.   12112023 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (low glucose) with 10% newborn calf serum (NCS; Biowest Cat. # S0750, Lot S14368S0750) Temperature   37 C
Cell Density at Seeding   1.6 - 4.9 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Never allow culture to become full confluency.
Doubling Time   16 - 20 hrs. Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   5% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   95.2 Additional information   
Cell No.   JCRB9014 Cell Name   3T3 L1
LOT No.   12122023 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (low glucose) with 10% newborn calf serum (NCS; Biowest Cat. # S0750, Lot S14368S0750) Temperature   37 C
Cell Density at Seeding   1.6 - 4.9 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Never allow culture to become full confluency.
Doubling Time   16 - 20 hrs. Cell Number in Vial (cells/1ml)   2.8 x 10^6
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   5% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   90.9 Additional information   
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