JCRB9020 BHK-21(C-13)

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9020 Cell Name   BHK-21(C-13)
Profile   kidney, subclone of BHK-21 Other Name   BHK
Animal   hamster, Syrian Strain   
Genus   Mesocricetus Species   auratus
Sex    Age   1-day-old
Identity    Tissue for Primary Cancer   kidney
Case history    Metastasis   
Tissue Metastasized    Genetics   kidney
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   kidney
Classify    Established by   Macpherson & Stoker
Registered by   Mizusawa,H. Regulation for Distribution   
Comment   Prepare from CCL10. Difco tryptose phosphate broth 29.5 g/l. Year   1985
Medium   Eagle's minimal essential medium with 10% calf serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   split ratio=1/2 to 1/10 Race   
CO2 Conc.   5 % Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1915
Reference
Pubmed id:14468055Polyoma transformation of hamster cell clones--an investigation of genetic factors affecting cell competence.
MACPHERSON I,STOKER M
Virology. 1962 Feb;16():147-51

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LOT Information

Cell No.   JCRB9020 Cell Name   
LOT No.   04022010 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non essential amino acids (0.1 mM each) and 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 22 hrs Cell Number in Vial (cells/1ml)   3.9 x 10^6
Viability at cell freezing (%)   89.2 Antibiotics Used   free
Passage Number   P60 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as Syrian hamster by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9020 Cell Name   
LOT No.   10012004 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% calf serum Temperature   37 C
Cell Density at Seeding   6-9 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   ca. 30 hrs Cell Number in Vial (cells/1ml)   2.8 x 10^6
Viability at cell freezing (%)   97.1 Antibiotics Used   free
Passage Number   P60 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as hamster(Syrian) by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9020 Cell Name   BHK-21(C-13)
LOT No.   100796 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9020 Cell Name   BHK-21(C-13)
LOT No.   072092 Lot Specification   distribution
Medium   BME with 10% Triptose phosphate(Difco)+10% CS(C.C.L.lot.00021-07) Filtered through 0.20 u membrane. Temperature   37 C
Cell Density at Seeding   8.18x10^5 Methods for Passages   Monolayers are washed with PBS(-) & treated with 0.25%trypsin-0.02%EDTA. Centrifuge in growth med.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.12x10^6
Viability at cell freezing (%)   90.6 Antibiotics Used   free
Passage Number   P57 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9020 Cell Name   BHK-21(C-13)
LOT No.   081386 Lot Specification   distribution
Medium   BME 80%, Difco tryptose phosphate broth 10%, CS10% Temperature   37
Cell Density at Seeding   1:2 to 1:10 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.2x10^6
Viability at cell freezing (%)   98% Antibiotics Used   free
Passage Number   P57 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9020 Cell Name   BHK-21(C-13)
LOT No.   06172020 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 30 hrs. Cell Number in Vial (cells/1ml)   5.0 x 10^6
Viability at cell freezing (%)   93.6 Antibiotics Used   free
Passage Number   P60 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - basal culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   83.1 Additional information   
Cell No.   JCRB9020 Cell Name   BHK-21(C-13)
LOT No.   09272023 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; GIBCO Cat. # 10437, Lot 1072322) Temperature   
Cell Density at Seeding   1 - 3 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   6.5 x 10^6
Viability at cell freezing (%)   77 Antibiotics Used   free
Passage Number   P60 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   73.8 Additional information   
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