JCRB9021 U937

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9021 Cell Name   U937
Profile   lymphoma, diffuse histiocytic, monocyte-like characteristics Other Name   U-937
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   37
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   diffuse histiocytic lymphoma Metastasis   
Tissue Metastasized    Genetics   express many monocyte-like characteristics
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Receptors for Fc & C3.Differentiation by chemicals
Classify   tumor Established by   Sundstrom & Nilsson
Registered by   Mizusawa,H. Regulation for Distribution   
Comment   Prepared from CRL1593. Year   1985
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Dilution. Optimal densities are between 0.5 and 1.0 x 10^6/ml.
Cell Number on Passage   0.1-1x10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   pleural effusion
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0007
Reference
Pubmed id:7381208Chemotactically responsive and nonresposive forms of a continuous human monocyte cell line.
Fischer DG,Pike MC,Koren HS,Snyderman R
J Immunol. 1980 Jul;125(1):463-5
Pubmed id:1083890Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines.
Ralph P,Moore MA,Nilsson K
J Exp Med. 1976 Jun 1;143(6):1528-33
Pubmed id:178611Establishment and characterization of a human histiocytic lymphoma cell line (U-937).
Sundström C,Nilsson K
Int J Cancer. 1976 May 15;17(5):565-77

Images



LOT Information

Cell No.   JCRB9021 Cell Name   
LOT No.   03182009 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099, Lot 484375) Temperature   37 C
Cell Density at Seeding   0.6 - 1.4 x 10^5 cells/ml Methods for Passages   Simple dilution
Doubling Time   approx. 22 hrs Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   83.0 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9021 Cell Name   
LOT No.   05242005 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   1 x 10^5 cells/ml Methods for Passages   dilution
Doubling Time   approx. 20 hrs Cell Number in Vial (cells/1ml)   2.9 x 10^6
Viability at cell freezing (%)   95.1 Antibiotics Used   free
Passage Number   Unknown (P5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9021 Cell Name   
LOT No.   12072001 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   1-2 x 10^5 cells/ml Methods for Passages   dilution
Doubling Time   24 hrs at log phase Cell Number in Vial (cells/1ml)   2.1 x 10^6
Viability at cell freezing (%)   89.0 Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9021 Cell Name   U937
LOT No.   040695 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat inactivated fetal bovine serum. Temperature   37 C
Cell Density at Seeding   2x10^5 cells/ml Methods for Passages   simple dilution
Doubling Time   21.4 hrs Cell Number in Vial (cells/1ml)   1.54x10^6
Viability at cell freezing (%)   94.8 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB),LD,NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:10,12
D7S820:9,11
D16S539:12
VWA:14,15
TH01:6,10
AM:X
TPOX:8,11
CSF1PO:12
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9021 Cell Name   U937
LOT No.   040594 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (mixed) Temperature   37 C
Cell Density at Seeding   1.0x10^5 cells/ml Methods for Passages   dilution
Doubling Time   21.2 hrs Cell Number in Vial (cells/1ml)   6.3x10^6
Viability at cell freezing (%)   95.2 Antibiotics Used   free
Passage Number   *P5 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9021 Cell Name   U937
LOT No.   042792 Lot Specification   distribution
Medium   RPMI1640 + 10% FCS (lot#=Cell Cult.Lab.0099-1) Temperature   37 C
Cell Density at Seeding    Methods for Passages   Dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.1x10^6
Viability at cell freezing (%)   91.7 Antibiotics Used   free
Passage Number   *P5 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9021 Cell Name   U937
LOT No.   110191 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   0.1-1x10^6 Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.6x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9021 Cell Name   U937
LOT No.   091090 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum Temperature   37
Cell Density at Seeding   0.1-1x10^6 Methods for Passages   Dilution. Optimal densities are between 0.5 and 1.0 x 10^6/ml.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.7x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   *P5 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   60XXY Chromosome Information   58/2,59/4,60/37,61/5,74/1,97/1 (50 cells counted)
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   culture medium with 5% DMSO CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9021 Cell Name   U937
LOT No.   121589 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   0.1-1x10^6 Methods for Passages   Dilution. Optimal densities are between 0.5 and 1.0 x 10^6/ml.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.3x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   *P4 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9021 Cell Name   U937
LOT No.   100488 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   0.1-1x10^5/ml Methods for Passages   Dilution. Optimal densities are between 0.5 and 1.0 x 10^6/ml.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.6x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   *P5 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP,LD,G6PD
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9021 Cell Name   U937
LOT No.   041087 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   0.1-1x10^5/ml Methods for Passages   Dilution. Optimal densities are between 0.5 and 1.0 x 10^6/ml.
Doubling Time   NT Cell Number in Vial (cells/1ml)   10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9021 Cell Name   U937
LOT No.   060892 Lot Specification   distribution
Medium   RPMI1640(lot.051992) + 10% FBS (J.R.Scientific lot. DO 10400) Temperature   37 C
Cell Density at Seeding   2-5x10^5/plate Methods for Passages   Harvest by centrifuge. Inoculum size: 1x10^5/ml when thawed; 2-5x10^4/ml for subculture.
Doubling Time    Cell Number in Vial (cells/1ml)   4.34x10^6
Viability at cell freezing (%)   96.1 Antibiotics Used   free
Passage Number   *P4 PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9021 Cell Name   
LOT No.   092297 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   1-3 x 10^5 cells/ml Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   4.5 x 10^6
Viability at cell freezing (%)   96.0 Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9021 Cell Name   U937
LOT No.   01192015 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   1.3 - 2.0 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   D.T. = approx. 39 hrs Cell Number in Vial (cells/1ml)   3.2 x 10^6
Viability at cell freezing (%)   78 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12
D13S317:10,12
D7S820:9,11
D16S539:12
VWA:14,15
TH01:6,9.3
AM:X
TPOX:8,11
CSF1PO:12
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   78 Additional information   
Cell No.   JCRB9021 Cell Name   U937
LOT No.   11242017 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   3.2 - 5.9 x 10^4 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 19 hrs. Cell Number in Vial (cells/1ml)   3.8 x 10^6
Viability at cell freezing (%)   88.9 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   88.9 Additional information   
Cell No.   JCRB9021 Cell Name   U937
LOT No.   10122020 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO：Cat No.;11875-093) with 10% heat inactivated fetal bovine serum(SIGMA,Cat#172012,Lot#12J396) ) Temperature   37 C
Cell Density at Seeding   0.37-2.0x10^5 Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.2x10^6
Viability at cell freezing (%)   96.56 Antibiotics Used   free
Passage Number   p6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:10,12
D7S820:9,11
D16S539:12
VWA:14,15
TH01:6,9.3
AM:X
TPOX:8,11
CSF1PO:12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   78.1 Additional information   
Images
Cell No.   JCRB9021 Cell Name   U937
LOT No.   06132022 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   1.8 - 2.9 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 26 hrs. Cell Number in Vial (cells/1ml)   2.8 x 10^6
Viability at cell freezing (%)   82.1 Antibiotics Used   fre
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12
D13S317:10,12
D7S820:9,11
D16S539:12
VWA:14,15
TH01:6,9.3
AM:X
TPOX:8,11
CSF1PO:12
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   83.3 Additional information   
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