JCRB9025 CPA
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB9025 | Cell Name | CPA |
|---|---|---|---|
| Profile | pulmonary artery normal endothelial cell | Other Name | CPA |
| Animal | bovine | Strain | |
| Genus | Bos | Species | taurus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | vascular system | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | pulmonary artery endothelium | |
| Life Span | finite | Crisis PDL | |
| Morphology | Character | pulmonary artery endothelium | |
| Classify | normal | Established by | Ryan,U. |
| Registered by | Mizusawa,H. | Regulation for Distribution | |
| Comment | When subculture, small polygonal(endotherial) cells detach while larger(smooth muscle) cells remain adherent. 60% cells detach, suspend after centrifuge (7-800 rpm, 10min), and resuspend in fresh medium. | Year | 1985 |
| Medium | Medium 199 with 20% fetal calf serum. | Methods for Passages | Cells are treated with 0.02 EDTA and 0.05% trypsin. |
| Cell Number on Passage | split ratio=1/3 - 1/4 | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | pulmonary artery |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:2507490 | Epidermal growth factor receptor-mediated selective cytotoxicity of antitumor agents toward human xenografts and murine syngeneic solid tumors. Amagase H,Kakimoto M,Hashimoto K,Fuwa T,Tsukagoshi S Jpn J Cancer Res. 1989 Jul;80(7):670-8 |
| Pubmed id:83028 | Isolation and culture of pulmonary artery endothelial cells. Ryan US,Clements E,Habliston D,Ryan JW Tissue Cell. 1978;10(3):535-54 |
LOT Information
| Cell No. | JCRB9025 | Cell Name | CPA |
|---|---|---|---|
| LOT No. | 010890 | Lot Specification | distribution |
| Medium | Medium 199 + FCS 20% | Temperature | 37 |
| Cell Density at Seeding | 1/3 - 1/4 | Methods for Passages | 0.02 EDTA and 0.05% trypsin. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.0x10^6 |
| Viability at cell freezing (%) | NT | Antibiotics Used | free |
| Passage Number | P24 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | NT |
| Sterility: FUNGI | NT | Isozyme Analysis | NT |
| Chromosome Mode | Chromosome Information | NT | |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB9025 | Cell Name | CPA |
|---|---|---|---|
| LOT No. | 091985 | Lot Specification | distribution |
| Medium | Medium 199 + FCS 20% | Temperature | 37 |
| Cell Density at Seeding | 1/3 - 1/4 | Methods for Passages | 0.02 EDTA and 0.05% trypsin. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2x10^6 |
| Viability at cell freezing (%) | NT | Antibiotics Used | free |
| Passage Number | P18 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | Chromosome Information | NT | |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB9025 | Cell Name | |
|---|---|---|---|
| LOT No. | 070298 | Lot Specification | distribution |
| Medium | Medium 199 with 20% fetal calf serum (FCS lot; BioWhittaker 6M0121) | Temperature | 37 C |
| Cell Density at Seeding | 1.0-1.5 x 10^5 cells/ml | Methods for Passages | 0.05% trypsin and 0.02% EDTA (0.25% trypsin and 0.02% EDTA was used in this lot praparation) |
| Doubling Time | 82 hrs | Cell Number in Vial (cells/1ml) | 6.0 x 10^5 |
| Viability at cell freezing (%) | 95.0 | Antibiotics Used | free |
| Passage Number | P22 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as bovine by NP, G6PD, AST. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |