JCRB9028 MDBK (NBL-1)

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9028 Cell Name   MDBK (NBL-1)
Profile   established from normal kidney Other Name   MDBK
Animal   bovine Strain   
Genus   Bos Species   taurus
Sex    Age   
Identity    Tissue for Primary Cancer   kidney
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   kidney, BVDV detected in the JCRB cell bank.
Classify    Established by   Madin,S.H.&Darby,N.B
Registered by    Regulation for Distribution   
Comment   Prepare from CCL 22. Year   1986
Medium   Eagle's minimal essential medium with Earle's BSS with 10 % fetal bovine serum Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   split ratio=1/2 - 1/4 Race   
CO2 Conc.   5 % Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0421
Reference
Pubmed id:3169015Microtubule-assembly inhibitor protein. Its distribution, localization and physicochemical properties.
Kotani S,Ikai A,Kawai G,Maekawa S,Yokoyama S,Sakai H
Eur J Biochem. 1988 Oct 1;176(3):573-80
Pubmed id:3137084Isolation of 110K actin binding protein from mammalian brain and its immunocytochemical localization within cultured cells.
Maekawa S,Sakai H
Exp Cell Res. 1988 Sep;178(1):127-42
Pubmed id:3481365Comparison of a major heat-stable microtubule-associated protein in HeLa cells and 190-kDa microtubule-associated protein in bovine adrenal cortex.
Murofushi H,Kotani S,Aizawa H,Maekawa S,Sakai H
J Biochem. 1987 Nov;102(5):1101-12




LOT Information

Cell No.   JCRB9028 Cell Name   
LOT No.   032699 Lot Specification   distribution
Medium   Eagle's minimal essential medium + 10% fetal calf serum (Biowhittaker 6M0121) Temperature   37℃
Cell Density at Seeding   1.0 x 10^5 cells/ml Methods for Passages   0.25%trypsin and 0.02% EDTA
Doubling Time   ca 24hrs Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability at cell freezing (%)   87% Antibiotics Used   free
Passage Number   P118 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as bovine by NP, G6PD, MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9028 Cell Name   MDBK (NBL-1)
LOT No.   083191 Lot Specification   distribution
Medium   Ham F-12 + FBS 10 % (heat inactivated FBS) Temperature   37 C
Cell Density at Seeding   1.4x10^6 Methods for Passages   0.02 % EDTA + 0.2 % trypsin
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.86x10^6
Viability at cell freezing (%)   99% Antibiotics Used   free
Passage Number   P119 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9028 Cell Name   MDBK (NBL-1)
LOT No.   07222019 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.8 - 4.2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   apprx. 31 hrs Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   93.5 Antibiotics Used   free
Passage Number   P118 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   94.8 Additional information   
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