JCRB9028 MDBK (NBL-1)
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB9028 | Cell Name | MDBK (NBL-1) |
|---|---|---|---|
| Profile | established from normal kidney | Other Name | MDBK |
| Animal | bovine | Strain | |
| Genus | Bos | Species | taurus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | kidney | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | kidney, BVDV detected in the JCRB cell bank. |
| Classify | Established by | Madin,S.H.&Darby,N.B | |
| Registered by | Regulation for Distribution | ||
| Comment | Prepare from CCL 22. | Year | 1986 |
| Medium | Eagle's minimal essential medium with Earle's BSS with 10 % fetal bovine serum | Methods for Passages | Cells are treated with 0.02% EDTA and 0.25% trypsin |
| Cell Number on Passage | split ratio=1/2 - 1/4 | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | kidney |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:3169015 | Microtubule-assembly inhibitor protein. Its distribution, localization and physicochemical properties. Kotani S,Ikai A,Kawai G,Maekawa S,Yokoyama S,Sakai H Eur J Biochem. 1988 Oct 1;176(3):573-80 |
| Pubmed id:3137084 | Isolation of 110K actin binding protein from mammalian brain and its immunocytochemical localization within cultured cells. Maekawa S,Sakai H Exp Cell Res. 1988 Sep;178(1):127-42 |
| Pubmed id:3481365 | Comparison of a major heat-stable microtubule-associated protein in HeLa cells and 190-kDa microtubule-associated protein in bovine adrenal cortex. Murofushi H,Kotani S,Aizawa H,Maekawa S,Sakai H J Biochem. 1987 Nov;102(5):1101-12 |
LOT Information
| Cell No. | JCRB9028 | Cell Name | |
|---|---|---|---|
| LOT No. | 032699 | Lot Specification | distribution |
| Medium | Eagle's minimal essential medium + 10% fetal calf serum (Biowhittaker 6M0121) | Temperature | 37℃ |
| Cell Density at Seeding | 1.0 x 10^5 cells/ml | Methods for Passages | 0.25%trypsin and 0.02% EDTA |
| Doubling Time | ca 24hrs | Cell Number in Vial (cells/1ml) | 2.6 x 10^6 |
| Viability at cell freezing (%) | 87% | Antibiotics Used | free |
| Passage Number | P118 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | confirmed as bovine by NP, G6PD, MD |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB9028 | Cell Name | MDBK (NBL-1) |
|---|---|---|---|
| LOT No. | 083191 | Lot Specification | distribution |
| Medium | Ham F-12 + FBS 10 % (heat inactivated FBS) | Temperature | 37 C |
| Cell Density at Seeding | 1.4x10^6 | Methods for Passages | 0.02 % EDTA + 0.2 % trypsin |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.86x10^6 |
| Viability at cell freezing (%) | 99% | Antibiotics Used | free |
| Passage Number | P119 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Exoteric Gene | NT | |
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB9028 | Cell Name | MDBK (NBL-1) |
|---|---|---|---|
| LOT No. | 07222019 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) | Temperature | 37 C |
| Cell Density at Seeding | 1.8 - 4.2 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | apprx. 31 hrs | Cell Number in Vial (cells/1ml) | 1.3 x 10^6 |
| Viability at cell freezing (%) | 93.5 | Antibiotics Used | free |
| Passage Number | P118 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 94.8 | Additional information |