JCRB9032 SJK-237-71
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB9032 | Cell Name | SJK-237-71 |
|---|---|---|---|
| Profile | anti-DNA polymerase alpha, IgG1 | Other Name | |
| Animal | mouse | Strain | |
| Genus | hybridoma | Species | |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | antibody-producing hybridoma | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | hybridoma cell line | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | producing monoclonal IgG1 to DNA polymerase alpha. |
| Classify | hybridoma | Established by | Korn, D. et al. |
| Registered by | Regulation for Distribution | ||
| Comment | Prepared from CRL1645. | Year | 1986 |
| Medium | Culbecco's modified Eagle's medium with 10% fetal calf serum. | Methods for Passages | Dilution. Maintain cultures at cell concentration between 10^5 and 10^6 per ml. |
| Cell Number on Passage | 1-2 x 10^5 cells/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | hybridoma |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:7045121 | Intracellular localization of human DNA polymerase alpha with monoclonal antibodies. Bensch KG,Tanaka S,Hu SZ,Wang TS,Korn D J Biol Chem. 1982 Jul 25;257(14):8391-6 |
| Pubmed id:7085672 | Preparation and preliminary characterization of monoclonal antibodies against human DNA polymerase alpha. Tanaka S,Hu SZ,Wang TS,Korn D J Biol Chem. 1982 Jul 25;257(14):8386-90 |
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LOT Information
| Cell No. | JCRB9032 | Cell Name | SJK-237-71 |
|---|---|---|---|
| LOT No. | 031997 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal bovine serum (JRH Biosciences, 1B1062). | Temperature | 37 C |
| Cell Density at Seeding | 2-3x10^4 cells/sq.cm. | Methods for Passages | Simple dilution. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.81x10^6 |
| Viability at cell freezing (%) | 96.7 | Antibiotics Used | free |
| Passage Number | P4 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO. | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
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