JCRB9033 STK1
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB9033 | Cell Name | STK1 |
|---|---|---|---|
| Profile | anti-DNA polymerase alpha, IgG1 | Other Name | |
| Animal | mouse | Strain | |
| Genus | Mus | Species | musclus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | antibody-producing hybridoma | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | hybridoma, monoclonal IgG1 to DNA polymerase alpha | |
| Life Span | Crisis PDL | ||
| Morphology | Character | hybridoma, monoclonal IgG1 to DNA polymerase alpha | |
| Classify | Established by | Korn, D. | |
| Registered by | Regulation for Distribution | ||
| Comment | Prejpared from CRL1652. | Year | 1986 |
| Medium | DMEM + FCS 10% | Methods for Passages | Dilution. Maintain cultures at cell concentration between 10^5-10^6/ml. |
| Cell Number on Passage | 1-2 x 10^5 cells/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | hybridoma |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:7045121 | Intracellular localization of human DNA polymerase alpha with monoclonal antibodies. Bensch KG,Tanaka S,Hu SZ,Wang TS,Korn D J Biol Chem. 1982 Jul 25;257(14):8391-6 |
| Pubmed id:7085672 | Preparation and preliminary characterization of monoclonal antibodies against human DNA polymerase alpha. Tanaka S,Hu SZ,Wang TS,Korn D J Biol Chem. 1982 Jul 25;257(14):8386-90 |
LOT Information
| Cell No. | JCRB9033 | Cell Name | STK1 |
|---|---|---|---|
| LOT No. | 070786 | Lot Specification | distribution |
| Medium | DMEM + FCS 10% | Temperature | 37 |
| Cell Density at Seeding | 1-2 x 10^5/ml | Methods for Passages | Dilution. Maintain cultures at cell concentration between 10^5-10^6/ml. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.4x10^6 |
| Viability at cell freezing (%) | NT | Antibiotics Used | free |
| Passage Number | P4* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | AST,G6PD,LD,MD,MPI,NP,PEPB |
| Chromosome Mode | Chromosome Information | NT | |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |