JCRB9035 CRFK

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9035 Cell Name   CRFK
Profile   kidney, BVD antigen negative Other Name   
Animal   cat Strain   
Genus   Felis Species   catus
Sex    Age   
Identity    Tissue for Primary Cancer   kidney
Case history    Metastasis   
Tissue Metastasized    Genetics   kidney
Life Span   infinite Crisis PDL   
Morphology    Character   kidney
Classify    Established by   
Registered by    Regulation for Distribution   Follow rules from ATCC
Comment   Prepared from CCL 94. Year   1986
Medium   Eagle's minimal essential medium with non essential amino acids and 10% fetal calf serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   split ratio=1/3 - 1/8 Race   
CO2 Conc.   5 % Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2426
Reference
Pubmed id:4130570Development, characterization, and viral susceptibility of a feline (Felis catus) renal cell line (CRFK).
Crandell RA,Fabricant CG,Nelson-Rees WA
In Vitro. 1973 Nov-Dec;9(3):176-85
Pubmed id:4930310Microbial studies of feline urolithiasis.
Fabricant CG,Rich LJ
J Am Vet Med Assoc. 1971 Mar 15;158(6):Suppl 2:976+


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ACS Omega. 2023 Nov 28;8(47):44839-44849

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LOT Information

Cell No.   JCRB9035 Cell Name   CRFK
LOT No.   071693 Lot Specification   distribution
Medium   Eagle's M.E.M. with non-essential A.A.(GIBCO,liquid,100x) and 10% FBS(J.R.H.Biosciences.lot# 1B1062) Temperature   37 C
Cell Density at Seeding   2x10^4 cells/sq.cm Methods for Passages   Cells are washed with PBS(-) then treated with 0.25% trypsin and 0.02% EDTA for 3 min. at 37 C.
Doubling Time   30.3 hr Cell Number in Vial (cells/1ml)   2.5x10^6
Viability at cell freezing (%)   98% Antibiotics Used   free
Passage Number   P186 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LDH, NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9035 Cell Name   CRFK
LOT No.   070593 Lot Specification   distribution
Medium   Eagle's minimal essential medium with NEAA and 10%FBS (Hyclone 1115975) Temperature   37 C
Cell Density at Seeding   2x10^4 cells/ml Methods for Passages   trypsin and EDTA Cells did not grow.
Doubling Time   NA Cell Number in Vial (cells/1ml)   NA
Viability at cell freezing (%)   NA Antibiotics Used   free
Passage Number   NA PDL   NA
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   NA
Chromosome Mode   NA Chromosome Information   NA
Surface Antigen   NA DNA Profile (STR)   
Adhesion   No Exoteric Gene   NA
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9035 Cell Name   CRFK
LOT No.   06032014 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   1.8 - 8.2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   77.0 Antibiotics Used   free
Passage Number   P187 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as cat by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   77.0 Additional information   
Cell No.   JCRB9035 Cell Name   CRFK
LOT No.   04112018 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.9 - 2.1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 25 hrs. Cell Number in Vial (cells/1ml)   3.3 x 10^6
Viability at cell freezing (%)   97.0 Antibiotics Used   free
Passage Number   P186 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM with NEAA CO2 Conc.   5%
Viability immediately after thawing (%)   97.0 Additional information   
Cell No.   JCRB9035 Cell Name   CRFK
LOT No.   06302021 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non essential amino acids and 10% fetal bovine serum (FBS; Biowest S1820-500) Temperature   37 C
Cell Density at Seeding   2.9 - 8.3 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   19 - 33 hrs. Cell Number in Vial (cells/1ml)   2.7 x 10^6
Viability at cell freezing (%)   95.7 Antibiotics Used   free
Passage Number   P186 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM with NEAA CO2 Conc.   5%
Viability immediately after thawing (%)   97.4 Additional information   
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