JCRB9038 EBTr (NBL-4)

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9038 Cell Name   EBTr (NBL-4)
Profile   embryonic trachea Other Name   
Animal   bovine Strain   
Genus   Bos Species   taurus
Sex    Age   
Identity    Tissue for Primary Cancer   lung
Case history    Metastasis   
Tissue Metastasized    Genetics   Embryonic bovine trachea
Life Span    Crisis PDL   
Morphology    Character   Embryonic bovine trachea
Classify    Established by   
Registered by    Regulation for Distribution   
Comment   Prepared from CCL 44. Year   
Medium   Minimal essential medium with non-essential amino acids and 10% new born calf serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   split ratio=1/2 - 1/4 Race   
CO2 Conc.   5 % Tissue Sampling   embryonic trachea
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2442
Reference
Pubmed id:2540915Binding of kinesin to stress fibers in fibroblasts under condition of microtubule depolymerization.
Okuhara K,Murofushi H,Sakai H
Cell Motil Cytoskeleton. 1989;12(2):71-7

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LOT Information

Cell No.   JCRB9038 Cell Name   EBTr (NBL-4)
LOT No.   012494 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% calf serum (Hyclone:2111480) Temperature   37 C
Cell Density at Seeding   1.1x10^5 cells/ml Methods for Passages   Cells are harvested with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.07x10^6
Viability at cell freezing (%)   95.7 Antibiotics Used   free
Passage Number   P34 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD, G6PD, NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9038 Cell Name   EBTr (NBL-4)
LOT No.   042093 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non essential amino acids and 10% calf serum Temperature   37 C
Cell Density at Seeding   6.8 x 10^4 cells/ml Methods for Passages   Cells are harvested by treatment with 0.25% trypsin and 0.01% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   7.3x10^5
Viability at cell freezing (%)   77.6 Antibiotics Used   free, This lot was discarded by the contamination.
Passage Number   P34 PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   +
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9038 Cell Name   EBTr (NBL-4)
LOT No.   01212019 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% newborn calf serum (NCS; Biowest Cat. # S0750) Temperature   37 C
Cell Density at Seeding   1.1 - 3.0 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 49 hrs. Cell Number in Vial (cells/1ml)   9.9 x 10^5
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   P34 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% newborn calf serum - basal culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   93.4 Additional information   
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