JCRB9040 PK(15)

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9040 Cell Name   PK(15)
Profile   kidney Other Name   
Animal   pig Strain   
Genus   Sus Species   scrofa
Sex    Age   
Identity    Tissue for Primary Cancer   kidney
Case history    Metastasis   
Tissue Metastasized    Genetics   Kidney
Life Span    Crisis PDL   
Morphology    Character   Kidney
Classify    Established by   Stice,E.
Registered by    Regulation for Distribution   
Comment   Prepared from CCL 33. Year   1986
Medium   Eagle's minimal essential medium with non-essential amino acids and 5% new born calf serum Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% Trypsin.
Cell Number on Passage   split ratio=1/2 - 1/4 Race   
CO2 Conc.   5 % Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2160
Reference
Pubmed id:4324256 C-type virus particles in pig kidney cell lines.
Armstrong JA,Porterfield JS,De Madrid AT
J Gen Virol. 1971 Feb;10(2):195-8
Pubmed id:4965860 Cytogenetic alterations in swine kidney cells persistently infected with hog cholera virus and propagated with and without antiserum in the medium.
Pirtle EC,Woods LK
Am J Vet Res. 1968 Jan;29(1):153-64

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LOT Information

Cell No.   JCRB9040 Cell Name   PK(15)
LOT No.   041387 Lot Specification   distribution
Medium   MEM,non essen amino acid,sodium pyruvate + NCS 5% Temperature   37
Cell Density at Seeding   1/2 - 1/4 Methods for Passages   0.02% EDTA and 0.25% Trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2x10^6
Viability at cell freezing (%)   95% Antibiotics Used   free
Passage Number   P136 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9040 Cell Name   
LOT No.   040999 Lot Specification   distribution
Medium   Eagle's MEM with non-essential amino acids, sodium pyruvate and 5% newborn calf serum Temperature   37 C
Cell Density at Seeding   0.7-1.9 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   P137 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as pig by NP, G6PD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9040 Cell Name   PK(15)
LOT No.   05192016 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 5% newborn calf serum (NCS; GIBCO Cat. # 16010) Temperature   37 C
Cell Density at Seeding   approx. 2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 20 hrs. Cell Number in Vial (cells/1ml)   3.5 x 10^6
Viability at cell freezing (%)   96.4 Antibiotics Used   free
Passage Number   P137 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   96.4
Additional information   
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