JCRB9047 GH3

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9047 Cell Name   GH3
Profile   pituitary tumor, somatotrophin and prolactin-secreting, (Wistar/Furth rat) Other Name   
Animal   rat Strain   Wistar/Furth
Genus   Rattus Species   norvegicus
Sex   F Age   
Identity    Tissue for Primary Cancer   pituitary
Case history    Metastasis   
Tissue Metastasized    Genetics   Pituitary tumor, MtT/W5, Somatotropin secreting.
Life Span    Crisis PDL   
Morphology    Character   Pituitary tumor, MtT/W5, Somatotrophin secreting.
Classify    Established by   Tashjian,A.H. et al.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CCL 82.1. Year   1986
Medium   Ham's F10 medium 82.5% with 15% horse serum and 2.5% fetal calf serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   split ratio=1/2 - 1/4 Race   
CO2 Conc.   5 % Tissue Sampling   pituitary gland
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0273
Reference
Pubmed id:4951281Establishment of clonal strains of rat pituitary tumor cells that secrete growth hormone.
Tashjian AH Jr,Yasumura Y,Levine L,Sato GH,Parker ML
Endocrinology. 1968 Feb;82(2):342-52




LOT Information

Cell No.   JCRB9047 Cell Name   
LOT No.   10152012 Lot Specification   distribution
Medium   Ham's F10 medium with 15% heat-inactivated horse serum (GIBCO 26050) and 2.5% fetal bovine serum (GIBCO 10099. Temperature   37 C
Cell Density at Seeding   3.2 - 8.5 x 10^5 clls/mL Methods for Passages   Pipetting, or 0.25% trypsin and 0.02% EDTA. Cell attachment is weak. Most of cells are non-adherent.
Doubling Time   74 - 156 hrs Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   82.4 Antibiotics Used   free
Passage Number   P30 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9047 Cell Name   GH3
LOT No.   101692 Lot Specification   distribution
Medium   Ham's F10 medium(Flow/Gibco) with 15% horse serum (JR Scientific, OD1223) and 2.5% FBS (Flow 047893) Temperature   37 C
Cell Density at Seeding   1.5x10^5 cells/ml Methods for Passages   Wash once with PBS(-) and treat with 0.25% trypsin and 0.02% EDTA for 10 min. at 37 C.
Doubling Time   26.8 hrs Cell Number in Vial (cells/1ml)   2.27x10^6
Viability at cell freezing (%)   86.8 Antibiotics Used   free
Passage Number   P29 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9047 Cell Name   GH3
LOT No.   050289 Lot Specification   distribution
Medium   Ham's F10, 82.5%; horse serum, 15%; FCS, 2.5%. Temperature   37
Cell Density at Seeding   1/2 - 1/4 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.8x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P29 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9047 Cell Name   
LOT No.   061797 Lot Specification   distribution
Medium   Ham's F10 medium with 15% horse serum and 2.5% fetal calf serum Temperature   37 C
Cell Density at Seeding   2-3 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Cell attachment is very weak and most cells grow as suspension.
Doubling Time   D.T.=ca 3 days Cell Number in Vial (cells/1ml)   2.1 x 10^6
Viability at cell freezing (%)   86.0 Antibiotics Used   free
Passage Number   P30 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as rat by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9047 Cell Name   GH3
LOT No.   10272022 Lot Specification   distribution
Medium   Ham's F-10 medium with 15% horse serum (GIBCO Cat. #16050) and 2.5% fetal bovine serum (FBS; GIBCO Cat. # 10437) Temperature   37 C
Cell Density at Seeding   6.3 - 7.5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Some cells may form cell aggregate and not adhere to the substratum.
Doubling Time   approx. 52 hrs. Cell Number in Vial (cells/1ml)   4.3 x 10^6
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   P29 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Y/N Exoteric Gene   
Medium for Freezing   10% DMSO in culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   96 Additional information   
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