JCRB9051 KG-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9051 Cell Name   KG-1
Profile   leukemia, acute myelogenous Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   59
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   
Tissue Metastasized    Genetics   bone marrow, acute myelogenous leukemia.
Life Span    Crisis PDL   
Morphology    Character   bone marrow, acute myelogenous leukemia.
Classify    Established by   Koeffler & Golde
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CCL246. Year   1986
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Dilution.
Cell Number on Passage   2-3x10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   bone marrow
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0374
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 907278
Reference
Pubmed id:6192693Fundamentals of chemotherapy of myeloid leukemia by induction of leukemia cell differentiation.
Hozumi M
Adv Cancer Res. 1983;38():121-69
Pubmed id:6996765Human myeloid leukemia cell lines: a review.
Koeffler HP,Golde DW
Blood. 1980 Sep;56(3):344-50
Pubmed id:6156728Mitogen stimulation of chronic lymphocytic leukemic lymphocytes: defective phytohemagglutinin stimulation independent of immunologic cell surface markers.
Davis S,Rambotti P
Blood. 1980 Aug;56(2):256-61
Pubmed id:306682Acute myelogenous leukemia: a human cell line responsive to colony-stimulating activity.
Koeffler HP,Golde DW
Science. 1978 Jun 9;200(4346):1153-4

Images



LOT Information

Cell No.   JCRB9051 Cell Name   
LOT No.   04252005 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   0.8 - 2.2 x 10^5 cells/ml Methods for Passages   Dilution
Doubling Time   35 - 50 hrs Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   79 Antibiotics Used   free
Passage Number   P32 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9051 Cell Name   KG-1
LOT No.   063095 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (ICN,Flow 0070893) Temperature   37 C
Cell Density at Seeding   1-3x10^5 cells/ml Methods for Passages   Cells are diluted and suspended into fresh medium.1-3 x 10^5 cells/ml at passage.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.7x10^6
Viability at cell freezing (%)   75.1 Antibiotics Used   free
Passage Number   P32 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:13
D13S317:11,12
D7S820:8,10
D16S539:10,11
VWA:14,19
TH01:7,8
AM:XY
TPOX:7,9
CSF1PO:7
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9051 Cell Name   
LOT No.   F00967 Lot Specification   distribution
Medium   RPMI1640 with 10% FCS Temperature   37
Cell Density at Seeding   1:3-1:5 Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   3.3 x 10^5
Viability at cell freezing (%)   75 Antibiotics Used   free
Passage Number   P33 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as hunam by isoenzymology
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9051 Cell Name   KG-1
LOT No.   083091 Lot Specification   distribution
Medium   RPMI1640 + FBS 10% Temperature   37
Cell Density at Seeding   2x10^6 Methods for Passages   dilution
Doubling Time   36 hr Cell Number in Vial (cells/1ml)   9.7x10^6
Viability at cell freezing (%)   89.2 Antibiotics Used   free
Passage Number   P34 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9051 Cell Name   KG-1
LOT No.   102387 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   2-3x10^5/ml Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   7x10^5
Viability at cell freezing (%)   91% Antibiotics Used   free
Passage Number   P31 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9051 Cell Name   KG-1
LOT No.   11122015 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% fetal bovine serum (FBS; GIBCO cat. # 10091) Temperature   37 C
Cell Density at Seeding   1.5 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time    Cell Number in Vial (cells/1ml)   3.8 x 10^5
Viability at cell freezing (%)   84 Antibiotics Used   free
Passage Number   P32 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI-1640 CO2 Conc.   5%
Viability immediately after thawing (%)   84 Additional information   
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