JCRB9053 L-2

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9053 Cell Name   L-2
Profile   adult lung Other Name   L-2
Animal   rat Strain   
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   lung
Case history    Metastasis   
Tissue Metastasized    Genetics   adult, lung, female
Life Span    Crisis PDL   
Morphology    Character   adult, lung, female
Classify    Established by   Kaighn and Douglas
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CCL 149. Year   1986
Medium   Ham's F12K medium with 10% fetal calf serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   split ratio=1/4 - 1/16 Race   
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0383
Reference
Pubmed id:1153240The choline incorporation pathway: primary mechanism for de novo lecithin synthesis in fetal primate lung.
Epstein MF,Farrell PM
Pediatr Res. 1975 Aug;9(8):658-65

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LOT Information

Cell No.   JCRB9053 Cell Name   L-2
LOT No.   081192 Lot Specification   distribution
Medium   Ham's F12K + fetal bovine serum (J.R.Scientific D10400, ICN Flow,1010183) Temperature   37 C
Cell Density at Seeding   7.2 x 10^5 cells/10D Methods for Passages   Cells were treated with 0.25% trypsin-0.02% EDTA then scraped with rubber policeman.
Doubling Time   NT Cell Number in Vial (cells/1ml)   9.3x10^6
Viability at cell freezing (%)   98.7 Antibiotics Used   free
Passage Number   P41 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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