JCRB9057 WI-38 VA13 sub 2 RA

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9057 Cell Name   WI-38 VA13 sub 2 RA
Profile   fetal lung, SV40 virus transformed, derivative of WI-38 Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   fetus
Identity   available Tissue for Primary Cancer   lung
Case history    Metastasis   
Tissue Metastasized    Genetics   lung, SV40 virus transformed cell line
Life Span   infinite Crisis PDL   
Morphology    Character   lung, SV40 virus transformed cell line, WI-38 derivative.
Classify   transformed Established by   Girardi, A.J. et al.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CCL 75.1. Year   
Medium   Eagle's minimal essential medium with non-essential amino acids, Na-pyruvate and 10% fetal bovine serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   1/2 - 1/10 Race   
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2759
Reference
Pubmed id:4290661 SV40 transformation of human diploid cells. A parallel study of viral and karyologic parameters.
Girardi AJ,Weinstein D,Moorhead PS
Ann Med Exp Biol Fenn. 1966;44(2):242-54

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LOT Information

Cell No.   JCRB9057 Cell Name   WI-38 VA13 sub 2 RA
LOT No.   041194 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential AAs,Na-pyruvate and 10% fetal bovine serum. Temperature   37 C
Cell Density at Seeding   8.2x10^5 cells/10cm dish Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA.Floating cells in culture medium are not viable.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.5x10^6
Viability at cell freezing (%)   90.0 Antibiotics Used   free
Passage Number   P268 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9057 Cell Name   WI-38 VA13 sub 2 RA
LOT No.   101190 Lot Specification   distribution
Medium   MEM,non essential amino acids,Na pyruvate + FCS10% Temperature   37
Cell Density at Seeding   1/2 - 1/10 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.4x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P269 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9057 Cell Name   WI-38 VA13 sub 2 RA
LOT No.   122586 Lot Specification   distribution
Medium   MEM,non essential amino acids,Na pyruvate + FCS10% Temperature   37
Cell Density at Seeding   1/2 - 1/10 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P228 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9057 Cell Name   WI-38 VA13 sub 2 RA
LOT No.   06132014 Lot Specification   distribution
Medium   Eagle's minimam essential medium with non-essential amino acids, sodium pyruvate and 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   2.6 - 6.7 x 10^4 cells/mL Methods for Passages   Cells are harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   D.T. = approx. 29 hrs Cell Number in Vial (cells/1ml)   3.8 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   P270 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10
D13S317:11
D7S820:9,11
D16S539:11,12
VWA:19,20
TH01:9.3
AM:X
TPOX:8
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   94
Additional information   
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