JCRB1210 MT-2
Cell information
Cell type：virus production cells (View Pricing Information)
| JCRB No. | JCRB1210 | Cell Name | MT-2 |
|---|---|---|---|
| Profile | Human cord leukocyte cell line established by co-cultivation with human ATL cells. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | infant |
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | human cord lymphocytes co-cultured with female ATL patient's lymphocytes. | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphoid | Character | HTLV-I positive. Type C particles observed. High level HTLV-1 producing cell line. |
| Classify | tumor | Established by | Miyoshi,I. |
| Registered by | Miyoshi,I. | Regulation for Distribution | |
| Comment | Year | 2007 | |
| Medium | RPMI 1640 medium with 20% fetal calf serum. | Methods for Passages | dilution |
| Cell Number on Passage | Split ratio = 1/2 - 1/3. | Race | Japanese |
| CO2 Conc. | 5% | Tissue Sampling | infant cord |
| Tissue Type | lymphocytes |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | + | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | + | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:6275274 | Type C virus particles in a cord T-cell line derived by co-cultivating normal human cord leukocytes and human leukaemic T cells. Miyoshi I,Kubonishi I,Yoshimoto S,Akagi T,Ohtsuki Y,Shiraishi Y,Nagata K,Hinuma Y Nature. 1981 Dec 24;294(5843):770-1 |
| Pubmed id:6281119 | A T-cell line derived from normal human cord leukocytes by co-culturing with human leukemic T-cells. Miyoshi I,Kubonishi I,Yoshimoto S,Shiraishi Y Gan. 1981 Dec;72(6):978-81 |
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LOT Information
| Cell No. | JCRB1210 | Cell Name | MT-2 |
|---|---|---|---|
| LOT No. | 09142007 | Lot Specification | distribution |
| Medium | RPMI1640 with 10% heat inactivated fetal bovine serum (Intergen RB52305) | Temperature | 37 C |
| Cell Density at Seeding | 5.5x10^5 cells/ml | Methods for Passages | suspension culture |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.6x10^6 |
| Viability at cell freezing (%) | 95.8 | Antibiotics Used | free |
| Passage Number | p10* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:13 D13S317:9,13 D7S820:8,13 D16S539:9,11 VWA:16 TH01:6,10 AM:X,Y TPOX:10,11 CSF1PO:10,11 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1210 | Cell Name | MT-2 |
|---|---|---|---|
| LOT No. | 03062018 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) | Temperature | 37 C |
| Cell Density at Seeding | NT | Methods for Passages | dilution(split ratio 1/2-1/3) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.5x10^6 |
| Viability at cell freezing (%) | 93.20 | Antibiotics Used | free |
| Passage Number | p11* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:13 D13S317:9,13 D7S820:8,13 D16S539:9,11 VWA:16 TH01:6,10 AM:X,Y TPOX:10,11 CSF1PO:10,11 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 67.7 | Additional information |
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| Cell No. | JCRB1210 | Cell Name | MT-2 |
|---|---|---|---|
| LOT No. | 06172021 | Lot Specification | distribution |
| Medium | RPMI1640 medium(Wako-FUJIFILM,#189-02025) with 10% heat inactivated fetal bovine serum(Biowest cat.No.S1820-500 LotNo.S09336S1820) | Temperature | 37 C |
| Cell Density at Seeding | 1.73-1.94x10^5 cells/ml | Methods for Passages | dilution |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 5.02x10^6 |
| Viability at cell freezing (%) | 93.8 | Antibiotics Used | free |
| Passage Number | p11* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:13 D13S317:9,13 D7S820:8,13 D16S539:9,11 VWA:16 TH01:6,10 AM:X,Y TPOX:10,11 CSF1PO:10,11 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 93.7 | Additional information | HTLV-1 posotive. Type C particles observed. High level HTLV-1 producing cell line. |
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