JCRB9062 HS-Sultan

Cell information

Important Notice(s)

JCB9062 HS Sultan: the problem of origin

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9062 Cell Name   HS-Sultan
Profile   established as plasmacytoma, but revealed as derivative of Jiyoye cell line (Burkitt's lymphoma) Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span    Crisis PDL   
Morphology    Character   
Classify   tumor Established by   Harris,N.S.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Originally thought as a plasma cytoma but later identified as a Burkitt's lymphoma. Year   1987
Medium   RPMI1640 medium with 10% Fetal calf serum. Methods for Passages   Dilution. A maximum of 3-4 x 10^6/ml is obtainable.
Cell Number on Passage   2-4 x 10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   Burkitt's lymphoma
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2516
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 907268
Reference
Pubmed id:321692Detection of plaque-forming cells in the peripheral blood of actively immunized humans.
Thomson PD,Harris NS
J Immunol. 1977 Apr;118(4):1480-2
Pubmed id:4549735Plasma cell surface antigen on human blood lymphocytes.
Harris NS
Nature. 1974 Aug 9;250(5466):507-9

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LOT Information

Cell No.   JCRB9062 Cell Name   
LOT No.   11162001 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   ca 8 x 10^4 cells/ml Methods for Passages   Dilution. (large aggregate can be dissociated into single cells by pipetting.)
Doubling Time    Cell Number in Vial (cells/1ml)   3.3 x 10^6
Viability at cell freezing (%)   64.8 Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12
D13S317:12
D7S820:8,10
D16S539:10,11
VWA:15,19
TH01:7,9
AM:X,Y
TPOX:6,8
CSF1PO:10,11
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9062 Cell Name   HS-Sultan
LOT No.   032395 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat inactivated fetal bovine serum (JRH Bioscienses, 1B1062) Temperature   37 C
Cell Density at Seeding   2-3x10^6 cells/ml Methods for Passages   Simple dilution. Clusters of cells are dispersed by pipetting.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.13x10^7
Viability at cell freezing (%)   98.2 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:12
D7S820:8,10
D16S539:10,11
VWA:15,19
TH01:7,9
AM:X,Y
TPOX:6,8
CSF1PO:10,11
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9062 Cell Name   HS-Sultan
LOT No.   061387 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   2-4 x 10^5/ml Methods for Passages   Dilution. A maximum of 3-4 x 10^6/ml is obtainable.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.2x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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