JCRB9063 WIL2-NS
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB9063 | Cell Name | WIL2-NS |
|---|---|---|---|
| Profile | B lymphocyte line, from patient with hereditary spherocytic anemia, non-immunoglobulin secreting variant | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | B lymphocyte, non-secreting variant | |
| Life Span | Crisis PDL | ||
| Morphology | Character | B lymphocyte, non-secreting variant | |
| Classify | Established by | Levy,J.A. et al. | |
| Registered by | Regulation for Distribution | Following rules from ATCC | |
| Comment | Prepared from CRL8155. A maximum of 1-2 x 10^6 cells/ml is obtainable. | Year | 1987 |
| Medium | RPMI1640 medium with 10% fetal calf serum. | Methods for Passages | Dilution |
| Cell Number on Passage | 1-2 x 10^5 cells/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | lymphocyte |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | + | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | EBV’s BALF-5 gene fragment was detected by realtime PCR. However, the BZLF-1 gene that is responsible for the activation of EBV is not expressed in this cell line under standard culture condition and even under the virus-inducing conditions. EBV particles will not be produced by this cell line. | ||||||||
| Reference | |
|---|---|
| Pubmed id:804494 | Antibodies to cellular antigens in Sjögren's syndrome. Alspaugh MA,Tan EM J Clin Invest. 1975 May;55(5):1067-73 |
| Pubmed id:4323138 | Further characterization of the WI-L1 and WI-L2 lymphoblastoid lines. Levy JA,Buell DN,Creech C,Hirshaut Y,Silverberg H J Natl Cancer Inst. 1971 Mar;46(3):647-54 |
| Pubmed id:5673231 | Human lymphoblastoid lines from lymph node and spleen. Levy JA,Virolainen M,Defendi V Cancer. 1968 Sep;22(3):517-24 |
| Images |
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LOT Information
| Cell No. | JCRB9063 | Cell Name | |
|---|---|---|---|
| LOT No. | 08102000 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% fetal bovine serum | Temperature | 37 C |
| Cell Density at Seeding | 2-10 x 10^5 cells/ml | Methods for Passages | dilution |
| Doubling Time | Cell Number in Vial (cells/1ml) | 6.5 x 10^6 | |
| Viability at cell freezing (%) | 93 | Antibiotics Used | free |
| Passage Number | P7* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, MD |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB9063 | Cell Name | WIL2-NS |
|---|---|---|---|
| LOT No. | 103095 | Lot Specification | distribution |
| Medium | RPMI-1640 medium with 10% heat inactivated fetal bovine serum (ICN Flow 101083) | Temperature | 37 C |
| Cell Density at Seeding | 1.2x10^5/m | Methods for Passages | Cells are diluted to 1-3x10^5 cells/ml. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 6.74x10^6 |
| Viability at cell freezing (%) | 95.5 | Antibiotics Used | free |
| Passage Number | P5* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:12,13 D13S317:11 D7S820:9,12 D16S539:11,12 VWA:17,20 TH01:8,10 AM:XY TPOX:8,11 CSF1PO:11,12 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO. | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB9063 | Cell Name | WIL2-NS |
|---|---|---|---|
| LOT No. | 061387 | Lot Specification | distribution |
| Medium | RPMI1640 + FCS 10% | Temperature | 37 |
| Cell Density at Seeding | 1-2 x 10^5/ml | Methods for Passages | Dilution |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.9x10^6 |
| Viability at cell freezing (%) | 99% | Antibiotics Used | free |
| Passage Number | P4* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD,LD,NP |
| Chromosome Mode | Chromosome Information | NT | |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |