JCRB1217 MT-3
Cell information
Cell type：virus production cells (View Pricing Information)
| JCRB No. | JCRB1217 | Cell Name | MT-3 |
|---|---|---|---|
| Profile | Human cord leukocyte cell line established by co-cultivation with human ATL cells. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | |
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | Co-culture of ATL cells with human cord leukocytes. | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphoid | Character | |
| Classify | tumor | Established by | Miyoshi,I. |
| Registered by | Miyoshi,I. | Regulation for Distribution | |
| Comment | Year | 2007 | |
| Medium | RPMI 1640 medium with 20% fetal calf serum (original description). It is recommended to add IL-2 up to 200 units/mL. | Methods for Passages | dilution |
| Cell Number on Passage | Split ratio = 1/2 - 1/3. | Race | Japanese |
| CO2 Conc. | 5% | Tissue Sampling | peripheral blood |
| Tissue Type | lymphocytes |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | + | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | + | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
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LOT Information
| Cell No. | JCRB1217 | Cell Name | MT-3 |
|---|---|---|---|
| LOT No. | 04282011 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 20% heat inactivated fetal bovine serum(SIGMA027K03911) and IL-2(200-50 U/ml)(Roche Interleukin-2,human) | Temperature | 37 C |
| Cell Density at Seeding | 2.8-3.2x10^6 cells/ml | Methods for Passages | Dilution (split ratio=1/2-1/3) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 8.8x10^6 |
| Viability at cell freezing (%) | 94.6 | Antibiotics Used | free |
| Passage Number | p12* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11 D13S317:10,11 D7S820:10.3,12 D16S539:9,11 VWA:14,17 TH01:9 AM:X,Y TPOX:8,9 CSF1PO:10,12 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1217 | Cell Name | MT-3 |
|---|---|---|---|
| LOT No. | 05062011 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 20% heat inactivated fetal bovine serum(SIGMA027K03911) and IL-2(200-25 U/ml)(Roche Interleukin-2,human) | Temperature | 37 C |
| Cell Density at Seeding | 2.0-3.2x10^6 cells/ml | Methods for Passages | Dilution (split ratio=1/2-1/3) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 8.5x10^6 |
| Viability at cell freezing (%) | 90.4 | Antibiotics Used | free |
| Passage Number | p13* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11 D13S317:10,11 D7S820:10.3,12 D16S539:9,11 VWA:14,17 TH01:9 AM:X,Y TPOX:8,9 CSF1PO:10,12 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1217 | Cell Name | MT-3 |
|---|---|---|---|
| LOT No. | 05192008 | Lot Specification | distribution |
| Medium | RPMI1640(GIBCO) with 20% heat inactivated fetal bovine serum(Hyclone GRD0051) and non essential amino acids(1/100). | Temperature | 37 C |
| Cell Density at Seeding | 4.2x10^5 cells/ml | Methods for Passages | Cell Banker BLC-1(Nihon Zenyaku Industries) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | NT |
| Viability at cell freezing (%) | NT | Antibiotics Used | free |
| Passage Number | p13* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11 D13S317:10,11 D7S820:10.3,12 D16S539:9,11 VWA:14,17 TH01:9 AM:X,Y TPOX:8,9 CSF1PO:10,12 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
| Images |
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