JCRB9064 LC540

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9064 Cell Name   LC540
Profile   Leydig cell testicular tumor Other Name   
Animal   rat Strain   
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   testis
Case history    Metastasis   
Tissue Metastasized    Genetics   leudig cell testicular tumor
Life Span   infinite Crisis PDL   
Morphology    Character   leudig cell testicular tumor
Classify    Established by   Steinberger,A.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CCL 43. Year   1987
Medium   Eagle's minimal essential medium with NEAA + Na pyruvate + 10% fetal bovine serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   Split ratio=1/2-1/4 Race   
CO2 Conc.   5 % Tissue Sampling   testicular tumor
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3536
Reference
Pubmed id:5688092Transplantable Leydig cell tumors in Fischer rats: horomone responsivity and hormone production.
Jacobs BB,Huseby RA
J Natl Cancer Inst. 1968 Nov;41(5):1141-53

Images



LOT Information

Cell No.   JCRB9064 Cell Name   LC540
LOT No.   013196 Lot Specification   distribution
Medium   Eagle's minimal essential medium with NEAA, Na-pyruvate and 10% fetal bovine serum. Temperature   37 C
Cell Density at Seeding   1.3 x 10^6 cells/ml Methods for Passages   Wash cells with PBS(-), and treated with 0.25% trypsin and 0.02% EDTA for 3-10 min. at 37 C.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.1x10^6
Viability at cell freezing (%)   93.0 Antibiotics Used   free
Passage Number   P58 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP,LDH and G6PD (rat confirmed)
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9064 Cell Name   LC-540
LOT No.   060289 Lot Specification   distribution
Medium   MEM,non essential amino acids,Na pyruvate + FCS10% Temperature   37
Cell Density at Seeding   1/2 - 1/4 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P57 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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